Recombinant Saccharomyces cerevisiae Uncharacterized acyltransferase CST26 (CST26)

Code CSB-CF330841SVG
MSDS
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Source in vitro E.coli expression system
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Product Details

Target Names
CST26
Uniprot No.
Alternative Names
CST26; PSI1; YBR042C; YBR0412; 2-acyl-1-lysophosphatidylinositol acyltransferase; Acyl-CoA:lyso-PI acyltransferase; Chromosome stability protein 26; Phosphatidylinositol stearoyl incorporating protein 1
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-397
Target Protein Sequence
MLHQKIAHKVRKVVVPGISLLIFFQGCLILLFLQLTYKTLYCRNDIRKQIGLNKTKRLFI VLVSSILHVVAPSAVRITTENSSVPKGTFFLDLKKKRILSHLKSNSVAICNHQIYTDWIF LWWLAYTSNLGANVFIILKKSLASIPILGFGMRNYNFIFMSRKWAQDKITLSNSLAGLDS NARGAGSLAGKSPERITEEGESIWNPEVIDPKQIHWPYNLILFPEGTNLSADTRQKSAKY AAKIGKKPFKNVLLPHSTGLRYSLQKLKPSIESLYDITIGYSGVKQEEYGELIYGLKSIF LEGKYPKLVDIHIRAFDVKDIPLEDENEFSEWLYKIWSEKDALMERYYSTGSFVSDPETN HSVTDSFKINRIELTEVLILPTLTIIWLVYKLYCFIF
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Acyltransferase with lysophosphatidic acid acyltransferase (LPAAT) activity. Fatty acyl substrates include 18:0-acyl-CoA, 16:0-acyl-CoA, 17:0-acyl-CoA and 14:0-acyl-CoA. Responsible for the acyl-CoA-dependent introduction of saturated very long chain fatty acids (VLCFAs) into phosphatidylinositol, transferring saturated FAs with 18 to 26 carbon atoms. Responsible for the incorporation of stearate into phosphatidylinositol. Overexpression has an effect on chromosome stability. Regulates phosphorylation and expression of glycerol-3-phosphate acyltransferase SCT1.
Gene References into Functions
  1. PSI1 is responsible for the stearic acid enrichment that is characteristic of phosphatidylinositol in yeast. PMID: 19796168
Subcellular Location
Lipid droplet.
Protein Families
1-acyl-sn-glycerol-3-phosphate acyltransferase family
Database Links

KEGG: sce:YBR042C

STRING: 4932.YBR042C

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