Recombinant Schizosaccharomyces pombe Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit (SPCC338.15)

Code CSB-CF006594SXV
Product Type Transmembrane Protein
Size Pls inquiry other sizes
Uniprot No. O59866
Storage Buffer Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Species Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Sequence ARQTVLAVADHDIGGYSTFLQSLTDRDFDVKTSYIKDESAKLFEYGERLYDNLILLSSQSKSLGPVFSPKSLLEFVQSGGNLFVVAGSQLPEGIRELGRQLDMFLAERQSVVVDHFNHAEGSDDIILLDGISENPYIISDETRAAGPILYKGIGHYLGPNPQTQPILRGNPTSYIYNTKTEAEVSKNPWAAGTQLFLVSVLQSSTGERVGLSGSIDMLKDEYLSPQSPSFSKSNFLFARDLTNWVFQRKGVLQATNMTYGKVAEPLESRNASCYRIKDEMIFSIDISLLEDGQQTPYVADDVQLELIMLDPYYRVNLVPVPSDSQTSQHYEAVLVAPDHYGDFTFKIEYKRPGLTPIEEKSTFTLRQFFHNEFPRFLPHAYPYYASCFSVLGAFLLFCGIWLLQKPAKPVVPSAKKQN
Source in vitro E.coli expression system
Gene Names wbp1
Protein Names Recommended name: Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit Short name= Oligosaccharyl transferase 48 kDa subunit EC= 2.4.1.119 Alternative name(s): Oligosaccharyl transferase subunit beta
Expression Region 20-437
Tag Info N-terminal 10xHis-tagged
Protein Description Full Length of Mature Protein
Storage Store at -20°C, for extended storage, conserve at -20°C or -80°C.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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Function Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER) (By similarity).
Subcellular Location Endoplasmic reticulum, Membrane, Single-pass type I membrane protein
Protein Families DDOST 48 kDa subunit family
Database Links

KEGG: spo:SPCC338.15

STRING: 4896.SPCC338.15.1

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