Recombinant Schizosaccharomyces pombe Uncharacterized protein C4D7.11 (SPAC4D7.11)

Code CSB-CF517636SXV
Size Pls inquire
Source in vitro E.coli expression system
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Product Details

Target Names dsc4
Uniprot No. O14175
Alternative Names
dsc4; SPAC4D7.11; DSC E3 ubiquitin ligase complex subunit 4; Defective for SREBP cleavage protein 4
Species Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Expression Region 1-281
Target Protein Sequence MDTIVLDQRGEVFSFFRSLDMLCYAIIAQQYFQDPTVLLLLLKVFVQLSYLTPKPFSQLN ALPLFYPLLLNFLISLMVRMFFNLPTAGESLDGYLYGGSIINFIGEKNESSRIDFITSDL VLFCLQIFMALILIASNKRPTQASHIQASQSGLSNVDGDEEPSDLITEDSRDTQQGQRQE DLQRQLENERSRLIARFSHSLYSFQHGLSFGSPQNRNTPLQRQSADSYSTPVCIEVDSED WKDLVWKSQYATENANTNSINNSPLSSNTTGVPNSVLTNPI
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Component of the DSC E3 ubiquitin ligase complex which is required for the sre1 transcriptional activator proteolytic cleavage to release the soluble transcription factor from the membrane in low oxygen or sterol conditions. The complex plays also an important role in the multivesicular body (MVB) pathway and functions in a post-endoplasmic reticulum pathway for protein degradation.
Subcellular Location Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein.
Database Links

KEGG: spo:SPAC4D7.11

STRING: 4896.SPAC4D7.11.1

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