Name: KARS1 (ZMA-001 Biosimilar) Recombinant Monoclonal Antibody
Brand: CUSABIO
SKU: CSB-RA618973MB1HU

Product Details

Uniprot No.

Q15046

Target Names

KARS1

Alternative Names

ZMA001 research-grade biosimilar; ZMA 001 research-grade biosimilar ;KARS1 antibody; KARS antibody; KIAA0070 antibody; Lysine--tRNA ligase antibody; EC 2.7.7.- antibody; EC 6.1.1.6 antibody; Lysyl-tRNA synthetase antibody; LysRS antibody

Species Reactivity

Human

Immunogen

Recombinant Human KARS1 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

0.01M PBS，pH7.4

Form

Liquid

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Notes

Validation Status
Application-specific performance (e.g., in flow cytometry, ELISA, IHC or other assay formats) has not yet been experimentally verified by CUSABIO. Users are advised to determine the optimal working conditions empirically in their own assay systems.
Guaranteed Quality
① Antibody purity > 95% tested by SDS-PAGE.
② Endotoxin level < 0.1EU/ug tested by LAL method.

Lead Time

3-4 weeks

Description

This recombinant monoclonal antibody is developed as a research-grade biosimilar to reference antibody ZMA-001, targeting KARS1 (Lysyl-tRNA Synthetase 1). KARS1 is an aminoacyl-tRNA synthetase that catalyzes the attachment of lysine to its cognate tRNA molecule, a critical step in protein translation. Beyond its canonical role in translation, KARS1 participates in immune regulation through its secreted form, which acts as a signaling molecule in inflammatory responses. Mutations in KARS1 have been associated with Charcot-Marie-Tooth disease and other neurological disorders, while aberrant expression patterns have been observed in various cancers and autoimmune conditions.

This biosimilar antibody provides researchers with a reliable tool for investigating KARS1 function in cellular metabolism, protein synthesis regulation, and disease pathogenesis. It enables studies exploring the dual role of KARS1 in both housekeeping translation and extracellular signaling pathways. The antibody supports research into neurodegenerative diseases, cancer biology, and immune system dysregulation where KARS1 plays a significant role.

Usage

It is a non-therapeutic biosimilar antibody, owning the same variable region from the corresponding approved therapeutic antibody. In conclusion, it is a research-grade biosimilar antibody and expressed in mammalian cell, which can be directly used as positive controls in drug discovery or used for rapid verification of the biological functions of target protein.

Related Products

Target Background

Function

Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages. Catalyzes the synthesis of the signaling molecule diadenosine tetraphosphate (Ap4A), and thereby mediates disruption of the complex between HINT1 and MITF and the concomitant activation of MITF transcriptional activity.; (Microbial infection) Interacts with HIV-1 virus GAG protein, facilitating the selective packaging of tRNA(3)(Lys), the primer for reverse transcription initiation.

Gene References into Functions

The studies showed that mutations in KARS lead to a newly defined subtype of leukoencephalopathy associated with sensorineural hearing impairment. The combined effect of reduced aminoacylation and release of LysRS from the multiple-synthetase complex (MSC) likely underlies the pathogenesis of the KARS mutations identified in this study. PMID: 28887846
Using the TruSight One sequencing panel we identified two novel mutations in KARS. Both mutations, never reported previously, occur in a highly conserved region of the catalytic domain and displayed a dramatic effect on KARS stability PMID: 27891585
Caspase-8 controls the secretion of inflammatory lysyl-tRNA synthetase in exosomes from colorectal cancer cells. PMID: 28611052
Results indicate that KRS can promote cell-cell and cellextracellular matrix adhesion for migration. PMID: 26891990
finding show that enzymatically active Shiga toxins trigger the dissociation of lysyl-tRNA synthetase (KRS) from the multi-aminoacyl-tRNA synthetase complex in human macrophage-like differentiated THP-1 cells and its subsequent secretion. PMID: 26643967
KRS at the plasma membrane plays new roles in metastatic migration as a signaling inducer, and causes intracellular signaling for cancer dissemination PMID: 26091349
tRK1 forms a complex with human enolases and interacts with tRK1 and human pre-lysyl-tRNA synthetase (preKARS2) PMID: 25918939
structural characteristics of the KRS-LR interaction on the cell surface PMID: 24983501
Lysyl-tRNA synthetase plays essential role in HIV replication, transcriptional regulation, cytokine-like signaling. [review] PMID: 23972532
The role of preKARS2 in the tRNA mitochondrial import. PMID: 23799079
The KARS variant is identified in two families affected by DFNB89-associated autosomal-recessive nonsyndromic hearing impairment. PMID: 23768514
C-terminal domain of HIV-1 capsid protein as surrogate for human lysyl tRNA synthetase PMID: 23208549
A single conformational change triggered by phosphorylation leads to multiple effects driving an exclusive switch of LysRS function from translation to transcription. PMID: 23159739
Dual role for motif 1 residues of human lysyl-tRNA synthetase in dimerization and packaging into HIV-1. PMID: 23095741
The work thus unveiled a unique function of KRS in the control of cell migration and its pathological implication in metastasis. PMID: 22751010
Data are consistent with hypothesis that maturation of cytoplasmic KARS precursor is needed to reveal the potent tRNA binding properties of mitochondrial KARS. PMID: 22235746
LysRS associates with the Pol domain of GagPol. PMID: 21763493
The results suggest that this unique geometry, which reconfigures the LysRS tetramer from alpha(2):alpha(2) to alpha(2)beta(1):beta(1)alpha(2), is designed to control both retention and mobilization of LysRS from the multi-tRNA synthetase complex PMID: 21536907
The interaction between helix 7 of LysRS and helix 4 of the capsid C-terminal domain of HIV-1 Gag (HIV-CA-CTD) was studied using circular dichromism spectroscopy and molecular dynamics simulation. PMID: 21093454
These results underscore the contribution of KARS to the emission of (one of) the principal signal(s) of immunogenic cell death, CRT exposure. PMID: 20699648
Loss-of-function lysyl-tRNA synthetase mutations associated with peripheral neuropathy and Charcot-Marie-Tooth disease. PMID: 20920668
HIV-1 Gag interacts with human lysyl-tRNA synthetase during viral assembly PMID: 12756246
packaged into human immunodeficiency virus type 1 (HIV-1) via its interaction with Gag; this enzyme facilitates the selective packaging of tRNA(3)(Lys), the primer for initiating reverse transcription, into HIV-1 PMID: 15220430
analysis of the interaction between HIV-1 Gag and human lysyl-tRNA synthetase PMID: 16702215
HIV-1 Vpr fulfills an essential role in the process of packaging of mitochondrial Lysyl-tRNA synthase. PMID: 17560997
This work reports further characterization of the interaction between HIV-1 capsid domain of Gag and human LysRS using truncation constructs and point mutations in the putative interaction helices. PMID: 17724017
present a 2.3-A crystal structure of a tetrameric form of human LysRS PMID: 18272479
mitoKARS is the first described member of a group of mitochondrial proteins whose interaction with mutant SOD1 contributes to mitochondrial dysfunction in ALS PMID: 18715867

Hide All

Involvement in disease

Charcot-Marie-Tooth disease, recessive, intermediate type, B (CMTRIB); Deafness, autosomal recessive, 89 (DFNB89)

Subcellular Location

[Isoform Cytoplasmic]: Cytoplasm, cytosol. Cytoplasm. Nucleus. Cell membrane; Peripheral membrane protein. Secreted.; [Isoform Mitochondrial]: Mitochondrion.

Protein Families

Class-II aminoacyl-tRNA synthetase family

Database Links

HGNC: 6215

OMIM: 601421

KEGG: hsa:3735

STRING: 9606.ENSP00000325448

UniGene: Hs.3100

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：
Please enter the characters shown. Click the image to refresh if unclear.

Code	CSB-RA618973MB1HU
Size	US$9799
	Order now
Have Questions?	Leave a Message or Start an on-line Chat

KARS1 (ZMA-001 Biosimilar) Recombinant Monoclonal Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background