KARS Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA618973MA1HU
Size	US$210
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Image	Western Blot Positive WB detected in: MCF-7 whole cell lysate(30µg), HepG2 whole cell lysate(30µg), Jurkat whole cell lysate(30µg), HeLa whole cell lysate(30µg) All lanes: KARS antibody at 1:1000 Secondary Goat polyclonal to Human IgG at 1/40000 dilution Predicted band size: 68 kDa Observed band size:72 kDa Exposure time: 15S Immunofluorescence staining of MCF-7 cell with CSB-RA618973MA1HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcγ fragment specific. Immunofluorescence staining of CACO-2 cell with CSB-RA618973MA1HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcγ fragment specific. Overlay Peak curve showing Raji cells stained with CSB-RA618973MA1HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcγ fragment specific at 1:200 dilution for 35 min at 4℃.Control antibody (green line) was human IgG1 (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Target Background

Product Details

Uniprot No.

Q15046

Target Names

KARS

Alternative Names

CMTRIB antibody; DFNB89 antibody; EC 6.1.1.6 antibody; KARS 1 antibody; KARS 2 antibody; KARS antibody; KARS1 antibody; KARS2 antibody; KIAA0070 antibody; KRS antibody; Lysine tRNA ligase antibody; Lysine--tRNA ligase antibody; LysRS antibody; Lysyl tRNA synthetase antibody; Lysyl-tRNA synthetase antibody; SYK_HUMAN antibody

Species Reactivity

Human

Immunogen

Recombinant Human KARS protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

hIgG1

Clone No.

10A11

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IF	1:50-1:200
FC	1:20-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages. Catalyzes the synthesis of the signaling molecule diadenosine tetraphosphate (Ap4A), and thereby mediates disruption of the complex between HINT1 and MITF and the concomitant activation of MITF transcriptional activity.; (Microbial infection) Interacts with HIV-1 virus GAG protein, facilitating the selective packaging of tRNA(3)(Lys), the primer for reverse transcription initiation.

Gene References into Functions

The studies showed that mutations in KARS lead to a newly defined subtype of leukoencephalopathy associated with sensorineural hearing impairment. The combined effect of reduced aminoacylation and release of LysRS from the multiple-synthetase complex (MSC) likely underlies the pathogenesis of the KARS mutations identified in this study. PMID: 28887846
Using the TruSight One sequencing panel we identified two novel mutations in KARS. Both mutations, never reported previously, occur in a highly conserved region of the catalytic domain and displayed a dramatic effect on KARS stability PMID: 27891585
Caspase-8 controls the secretion of inflammatory lysyl-tRNA synthetase in exosomes from colorectal cancer cells. PMID: 28611052
Results indicate that KRS can promote cell-cell and cellextracellular matrix adhesion for migration. PMID: 26891990
finding show that enzymatically active Shiga toxins trigger the dissociation of lysyl-tRNA synthetase (KRS) from the multi-aminoacyl-tRNA synthetase complex in human macrophage-like differentiated THP-1 cells and its subsequent secretion. PMID: 26643967
KRS at the plasma membrane plays new roles in metastatic migration as a signaling inducer, and causes intracellular signaling for cancer dissemination PMID: 26091349
tRK1 forms a complex with human enolases and interacts with tRK1 and human pre-lysyl-tRNA synthetase (preKARS2) PMID: 25918939
structural characteristics of the KRS-LR interaction on the cell surface PMID: 24983501
Lysyl-tRNA synthetase plays essential role in HIV replication, transcriptional regulation, cytokine-like signaling. [review] PMID: 23972532
The role of preKARS2 in the tRNA mitochondrial import. PMID: 23799079
The KARS variant is identified in two families affected by DFNB89-associated autosomal-recessive nonsyndromic hearing impairment. PMID: 23768514
C-terminal domain of HIV-1 capsid protein as surrogate for human lysyl tRNA synthetase PMID: 23208549
A single conformational change triggered by phosphorylation leads to multiple effects driving an exclusive switch of LysRS function from translation to transcription. PMID: 23159739
Dual role for motif 1 residues of human lysyl-tRNA synthetase in dimerization and packaging into HIV-1. PMID: 23095741
The work thus unveiled a unique function of KRS in the control of cell migration and its pathological implication in metastasis. PMID: 22751010
Data are consistent with hypothesis that maturation of cytoplasmic KARS precursor is needed to reveal the potent tRNA binding properties of mitochondrial KARS. PMID: 22235746
LysRS associates with the Pol domain of GagPol. PMID: 21763493
The results suggest that this unique geometry, which reconfigures the LysRS tetramer from alpha(2):alpha(2) to alpha(2)beta(1):beta(1)alpha(2), is designed to control both retention and mobilization of LysRS from the multi-tRNA synthetase complex PMID: 21536907
The interaction between helix 7 of LysRS and helix 4 of the capsid C-terminal domain of HIV-1 Gag (HIV-CA-CTD) was studied using circular dichromism spectroscopy and molecular dynamics simulation. PMID: 21093454
These results underscore the contribution of KARS to the emission of (one of) the principal signal(s) of immunogenic cell death, CRT exposure. PMID: 20699648
Loss-of-function lysyl-tRNA synthetase mutations associated with peripheral neuropathy and Charcot-Marie-Tooth disease. PMID: 20920668
HIV-1 Gag interacts with human lysyl-tRNA synthetase during viral assembly PMID: 12756246
packaged into human immunodeficiency virus type 1 (HIV-1) via its interaction with Gag; this enzyme facilitates the selective packaging of tRNA(3)(Lys), the primer for initiating reverse transcription, into HIV-1 PMID: 15220430
analysis of the interaction between HIV-1 Gag and human lysyl-tRNA synthetase PMID: 16702215
HIV-1 Vpr fulfills an essential role in the process of packaging of mitochondrial Lysyl-tRNA synthase. PMID: 17560997
This work reports further characterization of the interaction between HIV-1 capsid domain of Gag and human LysRS using truncation constructs and point mutations in the putative interaction helices. PMID: 17724017
present a 2.3-A crystal structure of a tetrameric form of human LysRS PMID: 18272479
mitoKARS is the first described member of a group of mitochondrial proteins whose interaction with mutant SOD1 contributes to mitochondrial dysfunction in ALS PMID: 18715867

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Involvement in disease

Charcot-Marie-Tooth disease, recessive, intermediate type, B (CMTRIB); Deafness, autosomal recessive, 89 (DFNB89)

Subcellular Location

[Isoform Cytoplasmic]: Cytoplasm, cytosol. Cytoplasm. Nucleus. Cell membrane; Peripheral membrane protein. Secreted.; [Isoform Mitochondrial]: Mitochondrion.

Protein Families

Class-II aminoacyl-tRNA synthetase family

Database Links

HGNC: 6215

OMIM: 601421

KEGG: hsa:3735

STRING: 9606.ENSP00000325448

UniGene: Hs.3100

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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