Name: ABCG8 Antibody
Brand: CUSABIO
SKU: CSB-PA875651LA01HU

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) ABCG8 Polyclonal antibody

Uniprot No.

Q9H221

Target Names

ABCG8

Alternative Names

ABCG8; ATP-binding cassette sub-family G member 8; Sterolin-2

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human ATP-binding cassette sub-family G member 8 protein (176-413AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The ABCG8 Antibody (Product code: CSB-PA875651LA01HU) is Non-conjugated. For ABCG8 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA875651LB01HU	ABCG8 Antibody, HRP conjugated	ELISA
FITC	CSB-PA875651LC01HU	ABCG8 Antibody, FITC conjugated
Biotin	CSB-PA875651LD01HU	ABCG8 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:2000-1:10000
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The product CSB-PA875651LA01HU is a polyclonal antibody raised in the rabbit against a partial recombinant protein mapping within 176-413 amino acids of human ABCG8 protein. It exists as the unconjugated IgG isoform. It can react with human and mouse samples. This ABCG8 antibody underwent protein G affinity chromatography purification, and its purity is greater than 95%. And it has verified its specificity and reliability in ELISA, WB, IHC, and IF applications.

Its target protein ABCG8 mainly regulates cholesterol metabolism by transporting sterols out of the liver and intestines and into the bile for excretion, a process critical for maintaining proper cholesterol homeostasis in the body and preventing the accumulation of cholesterol in the liver.

Target Background

Function

ABCG5 and ABCG8 form an obligate heterodimer that mediates Mg(2+)- and ATP-dependent sterol transport across the cell membrane. Plays an essential role in the selective transport of the dietary cholesterol in and out of the enterocytes and in the selective sterol excretion by the liver into bile. Required for normal sterol homeostasis. The heterodimer with ABCG5 has ATPase activity.

Gene References into Functions

we identified a novel mutation in the ABCG8 gene, which in the homozygous form was associated with generalized xanthomatosis, and in the heterozygous form was associated with isolated xanthelasmas PMID: 28739549
Case Reports: compound heterozygous for nonsense mutations in ABCG8 responsible for sitosterolemia. PMID: 28521186
ABCG8 genetic variants may have role in the development of cholelithiasis in patients with Gaucher disease type 1. PMID: 27981300
Genetic polymorphism within the ABCG8 gene is a risk factor for diabetes. PMID: 26088706
A polymorphism of the sterol transporter ABCG8 has been associated with the prevalence of end-stage renal disease PMID: 25804128
Mutation in ABCG8 is associated with sitosterolaemia. PMID: 25056759
A single nucleotide polymorphism of ABCG8 is associated with fasting plasma glucose levels in a cross-sectional study but do not predict hyperglycemia or incident type 2 diabetes. [meta-analysis] PMID: 23840693
The evolutionary conserved region of ABCG8 were found to be responsive to the Liver-X-Receptor. PMID: 23790976
Recurrence of gallstones after cholecystectomy is associated with ABCG8 genotype. PMID: 22869156
ABCG8 (and ABO) variants are associated with high intestinal cholesterol absorption and cardiovascular disease. PMID: 23707316
Both gallstone disease and p.D19H of ABCG8 are associated with diminished cholesterol absorption. PMID: 23406058
ABCG8-D19H variant associated with cholesterol gallstone disease PMID: 22898925
G574R variant is associated with moderately elevated plant sterol levels in Old Order Amish. Carriers of the 574R allele had modestly lower levels of carotid wall thickness compared with noncarriers. PMID: 23241408
The ABCG8 rs4148217 SNP is associated with serum TG, HDL-C and ApoA1 levels in our study populations, but this association is different between the Mulao and Han populations. PMID: 22548731
Data suggest that ABCG8 S107X heterozygous mutation affects plasma phytosterol levels but not cholesterol metabolism (i.e., intestinal absorption, biosynthesis). Mutation affects efficacy of phytosterols supplementation on cholesterol absorption. PMID: 22378727
ABCG8 D19H genotype was an important predictor of both symptomatic gallstone disease and biliary cancer. PMID: 21274884
In the present study, we observed a highly significant association of the ABCG8 DH genotype and H allele with gallstone susceptibility in the northern Indian population. PMID: 21039838
Associations of 4 common ABCG8 polymorphisms (D19H, Y54C, T400K, and A632V)with ischemic stroke and coronary artery disease were sought. There was a tendency toward reduced 54YY-genotype frequency among male patients under 50 years of age with stroke. PMID: 20854103
ABCG8 rs11887534, identified as a gallstone risk single-nucleotide polymorphism by whole genome scan, is also associated with an increased risk of biliary tract cancer PMID: 21062971
A systematic review and meta-analysis of ABCG8 polymorphisms and association with markers of cholesterol metabolism. PMID: 20581104
Common variants in ABCG8 and ABO are strongly associated with serum phytosterol levels and show concordant and previously unknown associations with coronary heart disease. PMID: 20529992
For the ABCG8 gene, the rs4148211 polymorphism was associated with higher plasma total cholesterol and LDLcholesterol concentrations in the total population. PMID: 20170916
Genetic variant 19H of ABCG8 is associated with coronary artery disease. PMID: 20592455
SNP D19H, but not SNP T400K, in the ABCG8 gene is significantly associated with GSD in an Indian population. PMID: 20594224
Twins carrying a heterozygous or homozygous ABCG8 D19H genotype have a significantly increased risk of gallstone disease. PMID: 20497293
strong association of sequence variants of HMGCR, SREBF1 and ABCG8 genes with the reduction of LDL-C after statin treatment in a Chinese population PMID: 20235787
Common DNA sequence polymorphisms in the ABCG8 gene contribute to heritable variation in the plasma concentrations of the plant sterols campesterol and sitosterol. PMID: 11893785
In a sitosterolemia patient a novel heterozygous mutation has been found in exon 5 of ABCG8 (c.584T>A; Leu195Gln). PMID: 12124998
Genetic variations in the ABCG8 gene may play a role in the genetic determination of plasma cholesterol levels and could possibly influence the gender-specific response of plasma cholesterol levels after dietary changes. PMID: 15311998
These findings indicate that the T400K polymorphism in ABCG8 may be associated with the incidence of gallstone disease in males. PMID: 17612515
The results of the genetic study taken together indicate that in gallstone-susceptible carriers of the ABCG8 19H allele, cholesterol cholelithiasis is secondary to increased hepatobiliary cholesterol secretion. PMID: 17626266
An association scan of >500,000 SNPs in individuals with gallstones and controls was performed; a follow-up study of the 235 most significant SNPs in affected individuals and controls replicated the disease association of SNP A-1791411 in ABCG8. PMID: 17632509
Single nucleotide polymorphisms in ABCG8 are associated with changes in cholesterol metabolism during weight loss PMID: 17827468
Upregulation of ABCG5/ABCG8 in gallstone patients, possibly mediated by increased liver X receptor alpha, may contribute to the cholesterol supersaturation of bile, a prerequisite for gallstone formation. PMID: 18007013
links between polymorphisms of ABC G8A (ABCG8) transporter gene to hypercholesterolemia and to gallstone disease risk (Review) PMID: 18522623
Coexistence of higher insulin resistance and hypercholesterolemia for carriers of the aspartate-19-histidine polymorphism may result in a greater risk of cardiovascular disease. PMID: 18581044
Genetic variation in the ABCG8 gene may influence the burden of atherosclerosis in familial hypercholesteremia. PMID: 18977479
The DH genotype and the H allele of the ABCG8 D19H polymorphism are associated with Gallbladder cancer susceptibility. PMID: 19018975
Insulin resistance elevates ABCG8 and increases susceptibility to cholesterol gallstones PMID: 19306529

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Involvement in disease

Gallbladder disease 4 (GBD4); Sitosterolemia (STSL)

Subcellular Location

Cell membrane; Multi-pass membrane protein. Apical cell membrane; Multi-pass membrane protein.

Protein Families

ABC transporter superfamily, ABCG family, Eye pigment precursor importer (TC 3.A.1.204) subfamily

Tissue Specificity

Predominantly expressed in the liver. Low expression levels in the small intestine and colon. Very low levels in other tissues, including brain, heart and spleen.

Database Links

HGNC: 13887

OMIM: 210250

KEGG: hsa:64241

STRING: 9606.ENSP00000272286

UniGene: Hs.413931

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Code	CSB-PA875651LA01HU
Size	US$166
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Image	Immunohistochemistry of paraffin-embedded human lung tissue using CSB-PA875651LA01HU at dilution of 1:100 Immunofluorescent analysis of MCF-7 cells using CSB-PA875651LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) Western Blot Positive WB detected in: Mouse heart tissue, Mouse lung tissue, Mouse kidney tissue, Mouse stomach tissue All lanes: ABCG8 antibody at 2.8μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 76 kDa Observed band size: 76 kDa
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ABCG8 Antibody

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