ACTRT1 Antibody

Datasheet

Code	CSB-PA001255LA01HU
Size	US$166
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Image	Immunohistochemistry of paraffin-embedded human colon tissue using CSB-PA001255LA01HU at dilution of 1:100 Immunohistochemistry of paraffin-embedded human gastric cancer using CSB-PA001255LA01HU at dilution of 1:100 Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA001255LA01HU at dilution of 1:100 Immunohistochemistry of paraffin-embedded human smooth muscle tissue using CSB-PA001255LA01HU at dilution of 1:100 Immunofluorescence staining of PC-3 cells with CSB-PA001255LA01HU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) ACTRT1 Polyclonal antibody

Uniprot No.

Q8TDG2

Target Names

ACTRT1

Alternative Names

ACTRT1 antibody; ARPT1 antibody; HSD27Actin-related protein T1 antibody; ARP-T1 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Actin-related protein T1 protein (201-376AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The ACTRT1 Antibody (Product code: CSB-PA001255LA01HU) is Non-conjugated. For ACTRT1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA001255LB01HU	ACTRT1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA001255LC01HU	ACTRT1 Antibody, FITC conjugated
Biotin	CSB-PA001255LD01HU	ACTRT1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Negatively regulates the Hedgehog (SHH) signaling. Binds to the promoter of the SHH signaling mediator, GLI1, and inhibits its expression.

Gene References into Functions

Study identified mutations in the ACTRT1 gene, which encodes actin-related protein T1 (ARP-T1), in two of the six families with Bazex-Dupre-Christol syndrome (BDCS) that were examined in this study. ARP-T1 was found to directly bind to the GLI1 promoter, thus inhibiting GLI1 expression, and loss of ARP-T1 led to activation of the Hedgehog pathway in individuals with BDCS. PMID: 28869610
Describes the discovery of two actin-related proteins as major components in a cytoskeletal, nonmotile structure of bull spermatozoa, suggesting that certain members of this family of proteins may serve functions other than nucleation of actin filaments. PMID: 12243744

Subcellular Location

Cytoplasm, cytoskeleton. Cytoplasm. Nucleus.

Protein Families

Actin family

Tissue Specificity

In skin, expressed in the basal, spinous and granular layers of the epidermis. Also expressed in hair follicles, sebaceaous glands, eccrine sweat glands and semen.

Database Links

HGNC: 24027

OMIM: 300487

KEGG: hsa:139741

STRING: 9606.ENSP00000360165

UniGene: Hs.680125

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Protein Service

Gene Synthesis Service

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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