ALKBH2 Antibody

Datasheet

Code	CSB-PA761219ESR2HU
Size	US$166
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Image	Western blot All lanes: Alpha-ketoglutarate-dependent dioxygenase alkB homolog 2 antibody at 2μg/ml + Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 30, 18 kDa Observed band size: 30 kDa Immunohistochemistry of paraffin-embedded human small intestine tissue using CSB-PA761219ESR2HU at dilution of 1:100 Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA761219ESR2HU at dilution of 1:100
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) ALKBH2 Polyclonal antibody

Uniprot No.

Q6NS38

Target Names

ALKBH2

Alternative Names

2OG Fe(II) oxy DC1 antibody; 9530023G02 antibody; ABH 2 antibody; ABH2 antibody; alkB alkylation repair homolog 2 antibody; ALKB2_HUMAN antibody; ALKBH2 alkB antibody; ALKBH2 antibody; Alkylated DNA repair protein alkB homolog antibody; Alkylated DNA repair protein alkB homolog 2 antibody; Alkylation repair homolog 2 antibody; Alpha ketoglutarate dependent dioxygenase alkB homolog 2 antibody; Alpha-ketoglutarate-dependent dioxygenase alkB homolog 2 antibody; AU016977 antibody; DNA oxidative demethylase ALKBH2 antibody; EC 1.14.11. antibody; FLJ99103 antibody; hABH2 antibody; mABH2 antibody; MGC90512 antibody; Oxy DC1 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human DNA oxidative demethylase ALKBH2 protein (1-261AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:200-1:1000
IHC	1:20-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Dioxygenase that repairs alkylated DNA and RNA containing 1-methyladenine and 3-methylcytosine by oxidative demethylation. Can also repair alkylated DNA containing 1-ethenoadenine (in vitro). Has strong preference for double-stranded DNA. Has low efficiency with single-stranded substrates. Requires molecular oxygen, alpha-ketoglutarate and iron.

Gene References into Functions

These results revealed that N3-ethylthymidine , but not other DNA lesions, could be repaired by Alkbh2 and Alkbh3 in mammalian cells. PMID: 26930515
studies reveal the ALKBH2 binding interface of PCNA and indicate that both germline and somatic ALKBH2 variants could have cellular effects on ALKBH2 function in DNA repair. PMID: 26408825
It was shown for first time that DNA glycosylase ALKBH2 can repair DNA adduct 1,N2-ethenoguanine. PMID: 25797601
ABH2 knockdown impairs rDNA transcription and leads to increased single-stranded and double-stranded DNA breaks in the rDNA genes. PMID: 23972994
Overexpression of ALKBH2 is associated with enhanced resistance to temozolomide in glioblastoma. PMID: 23258843
ALKBH2 is an upstream molecule of the oncoprotein, MUC1, and regulates cell cycle and EMT, resulting in progression of urothelial carcinomas. PMID: 23279696
combination of duplex interrogation and oxidation chemistry allows ALKBH2 to detect and process diverse lesions efficiently and correctly PMID: 22659876
The non-enzymatic binding of AAG to 3,N(4)-ethenocytosine specifically blocks ALKBH2-catalyzed repair of 3,N(4)-ethenocytosine but not that of methylated ALKBH2 substrates. PMID: 22079122
X-ray absorption spectroscopy structural investigation of early intermediates in the mechanism of DNA repair by human ABH2 PMID: 21510633
ABH2 is downregulated in a subset of gastric cancers, and might be involved in the molecular mechanism of gastric cancer through inhibiting the proliferation of gastric cancer cells. PMID: 21155885
This work has provided a detailed understanding of the structural features of the single-stranded DNA and double-stranded DNA preferences of ABH2 and ABH3. PMID: 20714506
Divergent sequences outside of the active site determine substrate specificities of ABH2. PMID: 20525795
Tumour protein (TP53) is directly involved by binding to the promoter of ALKBH2 in mediating photofrin-mediaated photodynamic therapy in U87 glioma cells. PMID: 20661249
first crystal structure of ABH2 PMID: 18432238
ABH2 is active in the direct reversal of epsilon A lesions, and that ABH2, together with the alkyl-N-adenine-DNA glycosylase, which is the most effective enzyme for the repair of epsilon A, comprise the cellular defense against epsilon A lesions PMID: 18519673
Mutations of ABH2 is associated with pediatric brain tumors. PMID: 19290481

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Subcellular Location

Nucleus. Note=Detected in replication foci during S-phase.

Protein Families

AlkB family

Tissue Specificity

Detected in colon, small intestine, ovary, testis, prostate, skeletal muscle, heart, liver and urinary bladder.

Database Links

HGNC: 32487

OMIM: 610602

KEGG: hsa:121642

STRING: 9606.ENSP00000343021

UniGene: Hs.374458

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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