Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

ALKBH2

Uniprot No.

Q6NS38

Research Area

Epigenetics and Nuclear Signaling

Alternative Names

2OG Fe(II) oxy DC1; 9530023G02 ; ABH 2; ABH2; alkB alkylation repair homolog 2; ALKB2_HUMAN; ALKBH2 alkB; ALKBH2; Alkylated DNA repair protein alkB homolog ; Alkylated DNA repair protein alkB homolog 2; Alkylation repair homolog 2; Alpha ketoglutarate dependent dioxygenase alkB homolog 2; Alpha-ketoglutarate-dependent dioxygenase alkB homolog 2; AU016977; DNA oxidative demethylase ALKBH2; EC 1.14.11.; FLJ99103; hABH2; mABH2; MGC90512 ; Oxy DC1

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

1-261aa

Target Protein Sequence

MDRFLVKGAQGGLLRKQEEQEPTGEEPAVLGGDKESTRKRPRREAPGNGGHSAGPSWRHIRAEGLDCSYTVLFGKAEADEIFQELEKEVEYFTGALARVQVFGKWHSVPRKQATYGDAGLTYTFSGLTLSPKPWIPVLERIRDHVSGVTGQTFNFVLINRYKDGCDHIGEHRDDERELAPGSPIASVSFGACRDFVFRHKDSRGKSPSRRVAVVRLPLAHGSLLMMNHPTNTHWYHSLPVRKKVLAPRVNLTFRKILLTKK
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.

Mol. Weight

56.3kDa

Protein Length

Full Length

Tag Info

N-terminal GST-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Tris-based buffer,50% glycerol

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Target Background

Function

Dioxygenase that repairs alkylated DNA and RNA containing 1-methyladenine and 3-methylcytosine by oxidative demethylation. Can also repair alkylated DNA containing 1-ethenoadenine (in vitro). Has strong preference for double-stranded DNA. Has low efficiency with single-stranded substrates. Requires molecular oxygen, alpha-ketoglutarate and iron.

Gene References into Functions

These results revealed that N3-ethylthymidine , but not other DNA lesions, could be repaired by Alkbh2 and Alkbh3 in mammalian cells. PMID: 26930515
studies reveal the ALKBH2 binding interface of PCNA and indicate that both germline and somatic ALKBH2 variants could have cellular effects on ALKBH2 function in DNA repair. PMID: 26408825
It was shown for first time that DNA glycosylase ALKBH2 can repair DNA adduct 1,N2-ethenoguanine. PMID: 25797601
ABH2 knockdown impairs rDNA transcription and leads to increased single-stranded and double-stranded DNA breaks in the rDNA genes. PMID: 23972994
Overexpression of ALKBH2 is associated with enhanced resistance to temozolomide in glioblastoma. PMID: 23258843
ALKBH2 is an upstream molecule of the oncoprotein, MUC1, and regulates cell cycle and EMT, resulting in progression of urothelial carcinomas. PMID: 23279696
combination of duplex interrogation and oxidation chemistry allows ALKBH2 to detect and process diverse lesions efficiently and correctly PMID: 22659876
The non-enzymatic binding of AAG to 3,N(4)-ethenocytosine specifically blocks ALKBH2-catalyzed repair of 3,N(4)-ethenocytosine but not that of methylated ALKBH2 substrates. PMID: 22079122
X-ray absorption spectroscopy structural investigation of early intermediates in the mechanism of DNA repair by human ABH2 PMID: 21510633
ABH2 is downregulated in a subset of gastric cancers, and might be involved in the molecular mechanism of gastric cancer through inhibiting the proliferation of gastric cancer cells. PMID: 21155885
This work has provided a detailed understanding of the structural features of the single-stranded DNA and double-stranded DNA preferences of ABH2 and ABH3. PMID: 20714506
Divergent sequences outside of the active site determine substrate specificities of ABH2. PMID: 20525795
Tumour protein (TP53) is directly involved by binding to the promoter of ALKBH2 in mediating photofrin-mediaated photodynamic therapy in U87 glioma cells. PMID: 20661249
first crystal structure of ABH2 PMID: 18432238
ABH2 is active in the direct reversal of epsilon A lesions, and that ABH2, together with the alkyl-N-adenine-DNA glycosylase, which is the most effective enzyme for the repair of epsilon A, comprise the cellular defense against epsilon A lesions PMID: 18519673
Mutations of ABH2 is associated with pediatric brain tumors. PMID: 19290481

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Subcellular Location

Nucleus. Note=Detected in replication foci during S-phase.

Protein Families

AlkB family

Tissue Specificity

Detected in colon, small intestine, ovary, testis, prostate, skeletal muscle, heart, liver and urinary bladder.

Database Links

HGNC: 32487

OMIM: 610602

KEGG: hsa:121642

STRING: 9606.ENSP00000343021

UniGene: Hs.374458

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Code	CSB-EP761219HU
MSDS	MSDS
Size	$224
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human DNA oxidative demethylase ALKBH2 (ALKBH2)

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