Product Details

Uniprot No.

Target Names

ARRDC3

Alternative Names

ARRDC3 antibody; KIAA1376 antibody; Arrestin domain-containing protein 3 antibody; TBP-2-like inducible membrane protein antibody; TLIMP antibody

Raised in

Rabbit

Species Reactivity

Human,Mouse,Rat

Immunogen

Synthesized peptide derived from the C-terminal region of Human ARRDC3.

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Form

Liquid

Tested Applications

WB, IHC, ELISA

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:100-1:300
ELISA	1:20000

Protocols

Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Background

Function

Adapter protein that plays a role in regulating cell-surface expression of adrenergic receptors and probably also other G protein-coupled receptors. Plays a role in NEDD4-mediated ubiquitination and endocytosis af activated ADRB2 and subsequent ADRB2 degradation. May recruit NEDD4 to ADRB2. Alternatively, may function as adapter protein that does not play a major role in recruiting NEDD4 to ADRB2, but rather plays a role in a targeting ADRB2 to endosomes.

Gene References into Functions

correlation analysis indicated that the expression of ARRDC3 was negatively correlated with ITGbeta4 in clinical prostate cancer (PCa) tissues and cell lines. Our data revealed that ARRDC3 can serve as a tumor suppressor to inhibit PCa progression and an independent marker to predict the risk of biochemical recurrence and metastasis after radical resection of PCa. PMID: 28782483
For the in vivo experiment, miR-182-5p overexpression also promoted the growth and progression of prostate cancer tumors. In this regard, we suggest that miR-182-5p may be a key androgen receptor-regulated factor that contributes to the development and metastasis of Chinese prostate cancers and may be a potential target for the early diagnosis and therapeutic studies of prostate cancer. PMID: 27109471
ARRDC3 functions as a switch to modulate the endosomal residence time and subsequent intracellular signaling of the beta2AR PMID: 27226565
We found that ARRDC3 is required for ALIX ubiquitination induced by activation of PAR1 PMID: 26490116
Promoter hypermethylation is an important mechanism of the transcriptional inactivation of ARRDC3 in invasive ductal breast carcinoma. PMID: 25148870
The ARRDC3 is one of six known human alpha-arrestins, and has been implicated in the downregulation of the beta2-adrenergic receptor (beta2AR). PMID: 25220262
High affinity binding of full-length ARRDC3 and Nedd4 is driven by the avid interaction of both PPXY motifs with either the WW2-WW3 or WW3-WW4 combinations. PMID: 24379409
Beta 2 adrenergic receptor endocytosis requires beta-arrestin2 but not ARRDC3. PMID: 23208550
single-nucleotide polymorphisms in ARRDC3, FLT1, and SKAP1 were significant predictors for survival androgen-deprivation therapy in prostate cancer patients. PMID: 21652578
The obesity risk locus contains a single gene, arrestin domain-containing 3 (ARRDC3), which interacts directly with beta-adrenergic receptors and is gender-specific. PMID: 21982743
Data show that ARRDC3 interacts with NEDD4 through two conserved PPXY motifs and recruits NEDD4 to the activated beta2-adrenergic receptor. PMID: 20559325
ARRDC3 directly binds to a phosphorylated form of ITGbeta4 leading to its internalization, ubiquitination and ultimate degradation, and suppress breast cancer progression. PMID: 20603614
[Thioredoxin-binding protein-2-like inducible membrane protein, TLIMP] TLIMP, a novel VD3- or PPARgamma ligand-inducible membrane-associated protein, plays a regulatory role in cell proliferation and PPARgamma activation. PMID: 16269462
Txnip regulates cellular metabolism independent of its binding to thioredoxin and the arrestin domains are crucial structural elements in metabolic functions of alpha-arrestin proteins PMID: 19605364

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Subcellular Location

Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Lysosome. Endosome. Early endosome.

Protein Families

Arrestin family

Tissue Specificity

Highly expressed in skeletal muscle, placenta, kidney, lung, liver, blood, adrenal gland, lymph node, mammary gland, thyroid, and trachea. Very low levels in colon, thymus, spleen, small intestine, bladder and bone marrow. Strong expression in differentia

Database Links

HGNC: 29263

OMIM: 612464

KEGG: hsa:57561

STRING: 9606.ENSP00000265138

UniGene: Hs.24684