Product Details

Uniprot No.

Target Names

BCCIP

Alternative Names

bccip antibody; BCCIP_HUMAN antibody; BCCIPalpha antibody; BCCIPbeta antibody; BRCA2 and CDKN1A interacting protein antibody; BRCA2 and CDKN1A-interacting protein antibody; BRCA2 and Cip1/p21 interacting protein antibody; Cdk inhibitor p21 binding protein antibody; P21 and CDK associated protein 1 antibody; P21- and CDK-associated protein 1 antibody; Protein TOK-1 antibody; Protein TOK1 antibody; TOK 1 antibody; TOK 1alpha antibody; TOK 1beta antibody; TOK1 antibody

Raised in

Rabbit

Species Reactivity

Human,Mouse,Rat

Immunogen

Human BCCIP

Immunogen Species

Homo sapiens (Human)

Isotype

IgG

Purification Method

Antigen Affinity purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

PBS with 0.1% Sodium Azide, 50% Glycerol, pH 7.3. -20°C, Avoid freeze / thaw cycles.

Tested Applications

ELISA,WB

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Background

Function

During interphase, required for microtubule organizing and anchoring activities. During mitosis, required for the organization and stabilization of the spindle pole. Isoform 2/alpha is particularly important for the regulation of microtubule anchoring, microtubule stability, spindle architecture and spindle orientation, compared to isoform 1/beta. May promote cell cycle arrest by enhancing the inhibition of CDK2 activity by CDKN1A. May be required for repair of DNA damage by homologous recombination in conjunction with BRCA2. May not be involved in non-homologous end joining (NHEJ).

Gene References into Functions

We analyzed the BCCIP protein level in 470 cases of human breast cancer to determine the associations between BCCIP and 53BP1, p53, and subtypes of breast cancer. We further constructed a unique BCCIP knockdown mouse model to determine whether a partial BCCIP deficiency leads to spontaneous breast cancer formation PMID: 29047390
BCCIPbeta, as a S7 modulator, contributes to the regulation of ribosomal and extraribosomal functions of S7 and has implications in cell growth and tumor development. PMID: 28510697
this study identifies BCCIP as a novel factor critical for microtubule regulation and explicates a mechanism utilized by BCCIP in tumor suppression. PMID: 28394342
Expression of BCCIP beta was notably higher in tumor tissues of esophageal squamous cell carcinoma (ESCC) and Eca 109 cells. In vitro studies such as starvation and refeeding assay along with BCCIP beta-shRNA transfection assay demonstrated that BCCIP beta expression promoted proliferation of ESCC cells. PMID: 27995408
we demonstrate that BCCIPbeta induces a conformational change within the RAD51 filament that promotes release of ADP to help maintain an active presynaptic filament. Our findings reveal a functional role for BCCIPbeta as a RAD51 accessory factor in HR. PMID: 27694622
The BCCIPbeta, but not BCCIPalpha, interacts with EIF6 and RPL23/UL14 and stabilizes the latter. PMID: 25150171
Results indicate that BCCIP germ line mutations are unlikely to be a major contributor to familial breast/ovarian cancer risk in our population. PMID: 23911796
In BRCA2, two novel frame shift mutations were identified as 5073-5074delCT and 6866delC. PMID: 11748848
BRCA2 germline mutations appear to have a milder clinical phenotype when compared to non-BRCA2 mutations, since survival is higher among breast cancer patients carrying a BRCA2 mutation compared to sporadic breast cancers. PMID: 11754111
Unique de novo mutation of BRCA2 has been identified in a woman with early onset breast cancer. PMID: 11836363
Overexpression of BCCIP beta delays the G1 to S progression and results in an elevated p21 expression PMID: 14726710
BCCIP is critical for BRCA2- and RAD51-dependent responses to DNA damage and homologous recombinational repair. PMID: 15713648
BCCIP in maintaining the transactivation activity of wild type p53; down-regulation of BCCIP as a novel mechanism to impair the p53 function in cells harboring wild type p53 PMID: 17135243
suggest an essential role of BCCIP in the maintenance of genomic integrity PMID: 17452982
BCCIP-dependent repair of double strand breaks by homologous recombination is an early RAD51 response to ionizing radiation-induced DNA damage. PMID: 18413737
LYRIC/AEG-1 is a negative regulator of BCCIPalpha, promoting proteasomal degradation either through direct interaction, or potentially through an indirect mechanism involving downstream effects of the NF-kappaB signaling pathway. PMID: 18440304
BCCIP is phosphorylated by the Src tyrosine kinase and dephosphorylated by the PTPmu tyrosine phosphatase; neurite outgrowth assays suggest that BCCIP and PTPmu are in a common signal transduction pathway. PMID: 18773424
BCCIP protein expression was not detectable in approximately 45% of all astrocytic tumors. PMID: 19653894
regulation of p21 intracellular distribution is a new mechanism for BCCIP to modulate p21 functions PMID: 19713748

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Subcellular Location

Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Cytoplasm, cytoskeleton, spindle pole.; [Isoform 1]: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole.; [Isoform 2]: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole.

Protein Families

BCP1 family

Tissue Specificity

Expressed at high levels in testis and skeletal muscle and at lower levels in brain, heart, kidney, liver, lung, ovary, pancreas, placenta, and spleen.

Database Links

HGNC: 978

OMIM: 611883

KEGG: hsa:56647

STRING: 9606.ENSP00000357748

UniGene: Hs.370292