Product Details

Uniprot No.

Target Names

CDC42BPA

Alternative Names

A930014J19Rik antibody; CDC42 binding protein kinase alpha (DMPK like) antibody; CDC42 binding protein kinase alpha antibody; CDC42 binding protein kinase alpha dmpk like antibody; CDC42 binding protein kinase beta antibody; CDC42-binding protein kinase alpha antibody; CDC42BPA antibody; DKFZp686L1738 antibody; DKFZp686P1738 antibody; DMPK like antibody; DMPK-like alpha antibody; FLJ23347 antibody; KIAA0451 antibody; MGC189823 antibody; MRCK alpha antibody; mrck antibody; mrcka antibody; MRCKA_HUMAN antibody; Myotonic dystrophy kinase related CDC42 binding protein kinase alpha antibody; Myotonic dystrophy kinase-related CDC42-binding kinase alpha antibody; Myotonic dystrophy protein kinase-like alpha antibody; OTTHUMP00000035727 antibody; OTTHUMP00000035728 antibody; OTTHUMP00000035729 antibody; OTTHUMP00000035730 antibody; pk428 antibody; RP5-1087E8.4 antibody; Ser thr protein kinase PK428 antibody; Ser thr protein kinase related to the myotonic dystrophy protein kinase antibody; Serine/threonine protein kinase MRCK alpha antibody; Serine/threonine-protein kinase MRCK alpha antibody

Raised in

Rabbit

Species Reactivity

Human,Mouse,Rat

Immunogen

Synthesized peptide derived from the Internal region of Human MRCKα.

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Form

Liquid

Tested Applications

WB, ELISA

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
ELISA	1:40000

Protocols

Western Blotting(WB) Protocol
ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Serine/threonine-protein kinase which is an important downstream effector of CDC42 and plays a role in the regulation of cytoskeleton reorganization and cell migration. Regulates actin cytoskeletal reorganization via phosphorylation of PPP1R12C and MYL9/MLC2. In concert with MYO18A and LURAP1, is involved in modulating lamellar actomyosin retrograde flow that is crucial to cell protrusion and migration. Phosphorylates: PPP1R12A, LIMK1 and LIMK2. May play a role in TFRC-mediated iron uptake. In concert with FAM89B/LRAP25 mediates the targeting of LIMK1 to the lamellipodium resulting in its activation and subsequent phosphorylation of CFL1 which is important for lamellipodial F-actin regulation. Triggers the formation of an extrusion apical actin ring required for epithelial extrusion of apoptotic cells.

Gene References into Functions

The authors describe the discovery of MRCKalpha as a downstream effector of apoptosis and trigger of epithelial extrusion. By describing this novel role of MRCKalpha during apoptosis, the authors cast light on the complex actomyosin cytoskeleton dynamics in the process of epithelial cell extrusion and, likely, in the homeostatic maintenance of tissues in multicellular organisms. PMID: 29162624
Cdc42BPA and Cdc42 signaling are important for colon cancer invasion, and Cdc42BPA has potential implications for colon cancer prognosis and treatment. PMID: 29072916
Silencing of CDC42BPA expression partially reduces miR29a3p inhibitor-induced migration and invasion of SW4807 cells PMID: 29039592
ICAP-1 monoubiquitylation helps in switching from ROCK2-mediated to MRCKalpha-mediated cell contractility. PMID: 28049720
Data show that the fusion gene cyclin D1/myotonic dystrophy kinase-related CDC42-binding protein kinase alpha (CCND1/MRCK) mRNA is resistant to CCND1-targeted miRNA regulation. PMID: 27025456
the functional relevance of the LRAP25-MRCK complex in LIMK1-cofilin signaling and the importance of LRAP adaptors as key determinants of MRCK cellular localization and downstream specificities. PMID: 25107909
MRCK participates in mediating the effects of HMI-1a3 on HeLa cell morphology. PMID: 24486483
A functional pathway involving PDK1-mediated activation of MRCKA, links EGF signaling to myosin contraction and directional migration. PMID: 25092657
Data show that chelerythrine blocks cellular activity of MRCK resulted in the specific loss of NM II-associated MLC phosphorylation in the lamella, and the consequential suppression of cell migration. PMID: 21457715
MRCKalpha takes part in transferrin (Tf)-iron uptake, probably via regulation of Tf- TfR endocytosis/endosome trafficking that is dependent on the cellular cytoskeleton. PMID: 20188707
Class II histone deacetylases are directly recruited by BCL6 transcriptional repressor PMID: 11929873
a Cdc42-MRCK signal mediates myosin-dependent cell motility and there is convergence between Rho and Cdc42 signalling PMID: 15723050
The level of MRCKalpha mRNA in various tissues strongly positively correlates with the level of TfR mRNA, indicating its possible role in the transferrin iron uptake pathway. PMID: 16412980
findings report that the Notch1 gene is a p53 target in human keratinocytes with a role in tumor suppression of this cell type through negative regulation of the ROCK1/2 and MRCKalpha kinases PMID: 17344417
analysis of the interaction of phorbol esters with the C1 domain of MRCK (myotonic dystrophy kinase-related Cdc42 binding kinase) alpha/beta PMID: 18263588

Hide All

Subcellular Location

Cytoplasm. Cell projection, lamellipodium.

Protein Families

Protein kinase superfamily, AGC Ser/Thr protein kinase family, DMPK subfamily

Tissue Specificity

Abundant in the heart, brain, skeletal muscle, kidney, and pancreas, with little or no expression in the lung and liver.

Database Links

HGNC: 1737

OMIM: 603412

KEGG: hsa:8476

STRING: 9606.ENSP00000355731

UniGene: Hs.35433

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Code	CSB-PA003269
Size	US$119
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Image	Western Blot analysis of K562 cells using MRCKα Polyclonal Antibody
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CDC42BPA Antibody

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Target Background