CEP76 Antibody

Datasheet

Code	CSB-PA855035LA01HU
Size	US$166
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Image	Western blot All lanes: CEP76 antibody at 0.5µg/ml + 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 75, 66, 30 kDa Observed band size: 75 kDa Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA855035LA01HU at dilution of 1:100 Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA855035LA01HU at dilution of 1:100
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) CEP76 Polyclonal antibody

Uniprot No.

Q8TAP6

Target Names

CEP76

Alternative Names

C18orf9 antibody; Centrosomal protein 76kDa antibody; Centrosomal protein of 76 kDa antibody; CEP 76 antibody; Cep76 antibody; CEP76_HUMAN antibody; Chromosome 18 open reading frame 9 antibody; FLJ12542 antibody; HsT1705 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Centrosomal protein of 76 kDa protein (241-550AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The CEP76 Antibody (Product code: CSB-PA855035LA01HU) is Non-conjugated. For CEP76 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA855035LB01HU	CEP76 Antibody, HRP conjugated	ELISA
FITC	CSB-PA855035LC01HU	CEP76 Antibody, FITC conjugated
Biotin	CSB-PA855035LD01HU	CEP76 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:20-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Centrosomal protein involved in regulation of centriole duplication. Required to limit centriole duplication to once per cell cycle by preventing centriole reduplication.

Gene References into Functions

Here, we found that centrosomal protein of 76 kDa (Cep76), previously shown to restrain centriole amplification, interacts with cyclin-dependent kinase 2 (CDK2) and is a bona fide substrate of this kinase. Cep76 is preferentially phosphorylated by cyclin A/CDK2 at a single site S83, and this event is crucial to suppress centriole amplification in S phase PMID: 27065328
Enforced Cep76 expression specifically inhibits centriole amplification in cells undergoing multiple rounds of duplication without preventing the formation of extra procentrioles from a parental template. PMID: 19460342

Subcellular Location

Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Note=Does not localize along the ciliary axoneme.

Protein Families

CEP76 family

Database Links

HGNC: 25727

KEGG: hsa:79959

STRING: 9606.ENSP00000262127

UniGene: Hs.236940

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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