CH25H Antibody

Code CSB-PA005307LA01HU
Size US$166
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  • Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA005307LA01HU at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA005307LA01HU at dilution of 1:100

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) CH25H Polyclonal antibody
Uniprot No.
Target Names
CH25H
Alternative Names
CH25H; Cholesterol 25-hydroxylase; Cholesterol 25-monooxygenase; h25OH
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human Cholesterol 25-hydroxylase protein (142-272AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The CH25H Antibody (Product code: CSB-PA005307LA01HU) is Non-conjugated. For CH25H Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA005307LB01HU CH25H Antibody, HRP conjugated ELISA
FITC CSB-PA005307LC01HU CH25H Antibody, FITC conjugated
Biotin CSB-PA005307LD01HU CH25H Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, IHC
Recommended Dilution
Application Recommended Dilution
IHC 1:20-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

CUSABIO uses the recombinant human CH25H protein (142-272AA) to immunize rabbits and then purify the rabbit antiserum through protein G to obtain the anti-CH25H antibody. This CH25H antibody is a polyclonal antibody and occurs as an unconjugated IgG. Its purity is greater than 95%. It only reacts with human CH25H protein, an IFN-induced enzyme that catalyzes the oxidation of cholesterol to 25-hydroxycholesterol (25HC) to reduce cholesterol accumulation. 25HC, a natural oxysterol, plays a role in regulating cholesterol homeostasis and sterol biosynthesis and exerts broad-spectrum antiviral function. And this anti-CH25H antibody can be used in ELISA and IHC for the detection of the CH25H protein.

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Target Background

Function
Catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes. Plays a key role in cell positioning and movement in lymphoid tissues: 25-hydroxycholesterol is an intermediate in biosynthesis of 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC), an oxysterol that acts as a ligand for the G protein-coupled receptor GPR183/EBI2, a chemotactic receptor for a number of lymphoid cells. May play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. As an interferon-stimulated gene, has broad antiviral activities against a wide range of enveloped viruses, such as vesicular stomatitis virus (VSV) and SARS coronavirus-2 (SARS-CoV-2). Its product, 25-hydroxycholesterol, activates the ER-localized enzyme ACAT to induce internalization of accessible cholesterol on the plasma membrane and restricts SARS-CoV-2 S protein-mediated fusion which inhibits virus replication. In testis, production of 25-hydroxycholesterol by macrophages plays a role in Leydig cell differentiation.
Gene References into Functions
  1. We examined whether 25HC restricts infection by mammalian reovirus. Results suggest that 25HC inhibits the efficiency of cellular entry of reovirus virions, which may require specific endosomal membrane dynamics for efficient membrane penetration. PMID: 29950420
  2. We detected Cholesterol 25-hydroxylase, the enzyme responsible for 25-HC production, in human spermatozoa at the level of the neck and the post acrosomal area. Upon incubation with spermatozoa, 25-HC induced calcium and cholesterol transients in connection with the acrosomal reaction. PMID: 27912195
  3. Exome analysis in 212 multiple sclerosis and 14 neuromyelitis optica patients identified a rare CH25H p.Q17H mutation in two NMO patients of Asian ancestry. In addition, association analysis of common CH25H variants. PMID: 26857497
  4. Infection with HCV causes up-regulation of interferon-inducible CH25H in vivo. PMID: 25999047
  5. These results demonstrate that CH25H constitutes a primary innate response against hepatitis C virus infection through regulating host lipid metabolism. PMID: 25903345
  6. Cholesterol 25-hydroxylase production by dendritic cells and macrophages is regulated by type I interferons. PMID: 20699362
  7. Based on the results of genome-wide screens, along with biological studies, we selected three genes as candidates for AD risk factors: ATP-binding cassette transporter A1 (ABCA1), cholesterol 25-hydroxylase (CH25H) and cholesterol 24-hydroxylase (CH24H). PMID: 16157450

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Subcellular Location
Endoplasmic reticulum membrane; Multi-pass membrane protein.
Protein Families
Sterol desaturase family
Database Links

HGNC: 1907

OMIM: 604551

KEGG: hsa:9023

STRING: 9606.ENSP00000360918

UniGene: Hs.47357

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