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CHKB Antibody

Datasheet
Code CSB-PA005354LA01HU
Size US$166
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Image
  • Western Blotting(WB) - CHKB Antibody

    Western Blot
    Positive WB detected in: Rat liver tissue, Mouse liver tissue
    All lanes: CHKB antibody at 6.6μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 46, 14 kDa
    Observed band size: 46 kDa

  • Immunohistochemistry(IHC) 1- CHKB Antibody

    IHC image of CSB-PA005354LA01HU diluted at 1:300 and staining in paraffin-embedded human thyroid tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence(IF) - CHKB Antibody

    Immunofluorescence staining of HepG2 cells with CSB-PA005354LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) CHKB Polyclonal antibody
Uniprot No.
Q9Y259
Target Names
CHKB
Alternative Names
CHETK antibody; Chetk-beta antibody; Chkb antibody; Chkb choline kinase beta antibody; Chkb protein antibody; CHKB_HUMAN antibody; CHKL antibody; Choline kinase beta antibody; Choline kinase-like antibody; Choline kinase-like protein antibody; Choline/ethanolamine kinase antibody; choline/ethanolamine kinase beta antibody; Choline/ethanolamine kinase; beta antibody; CK antibody; CK/EK antibody; CK/EK-beta antibody; CKB antibody; CKEKB antibody; EK antibody; EKB antibody; Ethanolamine kinase antibody; Ethanolamine kinase beta antibody; Ethanolamine kinase; beta antibody; MDCMC antibody
Raised in
Rabbit
Species Reactivity
Human, Rat, Mouse
Immunogen
Recombinant Human Choline/ethanolamine kinase protein (2-99AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The CHKB Antibody (Product code: CSB-PA005354LA01HU) is Non-conjugated. For CHKB Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA005354LB01HU CHKB Antibody, HRP conjugated ELISA
FITC CSB-PA005354LC01HU CHKB Antibody, FITC conjugated
Biotin CSB-PA005354LD01HU CHKB Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:100-1:300
IF 1:50-1:200
Protocols
ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Troubleshooting and FAQs
Antibody FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Related Products

CHKB antibodies

Customer Reviews and Q&A

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Target Background

Function
Has a key role in phospholipid metabolism, and catalyzes the first step of phosphatidylethanolamine and phosphatidylcholine biosynthesis.
Gene References into Functions
  1. findings suggest that CKbeta, in concert with CKalpha, and depending on its phosphorylation status, might play a critical role as a druggable target in carcinogenesis PMID: 27149373
  2. The whole exome sequencing revealed a novel homozygous missense mutation in the Choline Kinase-Beta (CHKB) gene in exon 9. PMID: 27123443
  3. A new form of congenital muscular dystrophy with multisystem involvement and characteristic mitochondrial structural changes, due to choline kinase beta gene defects has been characterized. PMID: 26067811
  4. A novel silent variant in the choline kinase beta causing muscular dystrophy. PMID: 25740612
  5. Its mutations cause congenital muscular dystrophy.[Review] PMID: 24291895
  6. study identified a novel haplotype consisting of the indel variation, which had not been detected in previous studies in Japanese and Korean populations, and observed four single-nucleotide polymorphisms in CHKB/CPT1B PMID: 24571861
  7. CHKB encodes choline kinase beta, an enzyme that catalyzes the first de-novo biosynthetic step of phosphatidylcholine, the most abundant phospholipid in the eukaryotic membrane [review] PMID: 23945283
  8. CHKB activity was reduced in all three patients, significant reduction in choline:N-acetyl aspartate and choline:creatine ratios in keeping with a general decrease in the amount of choline and phosphocholine-based substrate PMID: 23692895
  9. The study extends on the observation of a strong multiethnic association of polymorphisms in the TCRA and P2RY11 with narcolepsy, but does not confirm the association of CPT1B/CHKB (rs5770917) in the Chinese population. PMID: 22177342
  10. homozygous or compound heterozygous mutations in the gene encoding choline kinase beta were identified in individulas with congenital muscular dystrophy. PMID: 21665002
  11. A single nucleotide polymorphism located between CPT1B and CHKB, was associated with narcolepsy in Japanese (rs5770917[C], odds ratio (OR) = 1.79, combined P = 4.4 x 10(-7)) and other ancestry groups (OR = 1.40, P = 0.02). PMID: 18820697
  12. Japanese CNS hypersomnias (essential hypersomnia: EHS) other than narcolepsy with cataplexy was significantly associated with SNP rs5770917 (located between CPT1B and CHKB) and HLA-DRB1*1501-DQB1*0602 haplotype PMID: 19404393

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Protein Families
Choline/ethanolamine kinase family
Database Links

HGNC: 1938

OMIM: 612395

KEGG: hsa:1120

STRING: 9606.ENSP00000384400

UniGene: Hs.654827

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