COL8A1 Antibody

Datasheet

Code	CSB-PA005755LA01HU
Size	$600
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Image	Western Blot Positive WB detected in: A549 whole cell lysate,HepG2 whole cell lysate, Hela whole cell lysate, 293 whole cell lysate, U251 whole cell lysate All lanes:COL8A1 Antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size:73 kDa Observed band size:73 kDa IHC image of CSB-PA005755LA01HU diluted at 1:67 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody Immunofluorescence staining of Hela cell with CSB-PA005755LA01HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) COL8A1 Polyclonal antibody

Uniprot No.

P27658

Target Names

COL8A1

Alternative Names

C3orf7 antibody; CO8A1_HUMAN antibody; COL8A1 antibody; Collagen alpha 1(VIII) chain [Precursor] antibody; Endothelial collagen antibody; MGC9568 antibody; Vastatin antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Collagen alpha-1(VIII) chain protein (572-744AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The COL8A1 Antibody (Product code: CSB-PA005755LA01HU) is Non-conjugated. For COL8A1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA005755LB01HU	COL8A1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA005755LC01HU	COL8A1 Antibody, FITC conjugated
Biotin	CSB-PA005755LD01HU	COL8A1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen affinity purification

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.02% sodium azide
Constituents: PBS containing 50% glycerol pH 7.3

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
IF	1:20-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The COL8A1 polyclonal antibody was produced in rabbits using the recombinant human COL8A1 protein containing amino acids 572-744 as the immunogen. After immunization, the antibody was isolated and subjected to protein G chromatography to achieve a purity level exceeding 95%. This antibody can recognize and bind the COL8A1 protein from human and mouse samples.

This COL8A1 polyclonal antibody has been validated in various applications, including ELISA, WB, and IF. This demonstrates the ability of the COL8A1 polyclonal antibody to detect COL8A1 protein levels and distribution in these different contexts.

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Target Background

Function

Macromolecular component of the subendothelium. Major component of the Descemet's membrane (basement membrane) of corneal endothelial cells. Also component of the endothelia of blood vessels. Necessary for migration and proliferation of vascular smooth muscle cells and thus, has a potential role in the maintenance of vessel wall integrity and structure, in particular in atherogenesis.; Vastatin, the C-terminal fragment comprising the NC1 domain, inhibits aortic endothelial cell proliferation and causes cell apoptosis.

Gene References into Functions

COL8A1 was identified and proved to be correlated with the progression and prognosis of human colon adenocarcinoma, probably through regulating focal adhesion-related pathways. PMID: 29497907
High COL8A1 level is associated with chronic obstructive pulmonary disease and cancer. PMID: 27234597
The COL8A1 rs13095226 polymorphism is not associated with nAMD or PCV, which suggesting this gene maybe not a susceptibility gene locus for neovascular age-related macular degeneration (nAMD) or polypoidal choroidal vasculopathy (PCV) in Chinese subjects. PMID: 26617902
High COL8A1 expression is associated with early loss of kidney function. PMID: 26110394
Data indicate that complement factor H (CFH) R1210C and common variants in COL8A1 and RAD51B plus six genes contribute predictive information for advanced macular degeneration (AMD) beyond macular and behavioral phenotypes. PMID: 24498017
The results are consistent with the proposal that rare CNVs play a role in TS aetiology and suggest a possible role for rearrangements in the COL8A1 and NRXN1 gene regions. PMID: 23533600
In addition, eight genes classified as 'second tier' hits in the original study (PAX7, THADA, COL8A1/FILIP1L, DCAF4L2, GADD45G, NTN1, RBFOX3 and FOXE1) showed evidence of linkage and association in this replication sample. PMID: 23512105
The purpose of this study is to evaluate COL8A1 and COL8A2 as candidate genes for thin central corneal thickness in human primary open angle glaucoma patients. PMID: 21139683
The absence of pathogenic mutations identified in the COL8A1 or COL8A2 genes in affected members of 15 pedigrees with familial FECD (Fuchs endothelial corneal dystrophy) indicates that other genetic factors are involved. PMID: 16936088
The absence of pathogenic mutations in COL8A1 and COL8A2 in patients with keratoconus indicates that other genetic factors are involved in the pathogenesis of this corneal ectatic disorder. PMID: 17721297

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Subcellular Location

Secreted, extracellular space, extracellular matrix, basement membrane.

Tissue Specificity

Expressed primarily in the subendothelium of large blood vessels. Also expressed in arterioles and venules in muscle, heart, kidney, spleen, umbilical cord, liver and lung and is also found in connective tissue layers around hair follicles, around nerve b

Database Links

HGNC: 2215

OMIM: 120251

KEGG: hsa:1295

STRING: 9606.ENSP00000261037

UniGene: Hs.654548

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