EIF3I Antibody

Datasheet

Code	CSB-PA03105A0Rb
Size	US$166
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Image	Western Blot Positive WB detected in: Rat brain tissue All lanes: EIF3I antibody at 3.2µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 37 kDa Observed band size: 37 kDa Immunofluorescence staining of Hela cells with CSB-PA03105A0Rb at 1:266, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) EIF3I Polyclonal antibody

Uniprot No.

Q13347

Target Names

EIF3I

Alternative Names

eIF-3-beta antibody; eIF3 beta antibody; eIF3 p36 antibody; eIF3-beta antibody; eIF3-p36 antibody; eIF3i antibody; EIF3I_HUMAN antibody; EIF3S2 antibody; EIF3S2; formerly antibody; Eukaryotic translation initiation factor 3 subunit 2 antibody; Eukaryotic translation initiation factor 3 subunit 2 beta antibody; Eukaryotic translation initiation factor 3 subunit I antibody; eukaryotic translation initiation factor 3; subunit 2 (beta; 36kD antibody; eukaryotic translation initiation factor 3; subunit 2 beta; 36kDa antibody; eukaryotic translation initiation factor 3; subunit 2; formerly antibody; eukaryotic translation initiation factor 3; subunit I antibody; Predicted protein of HQ2242 antibody; PRO2242 antibody; TGF beta receptor interacting protein 1 antibody; TGF-beta receptor-interacting protein 1 antibody; TGFbeta receptor interacting protein 1 antibody; TGFbeta receptor-interacting protein 1 antibody; TRIP 1 antibody; TRIP-1 antibody; TRIP1 antibody

Raised in

Rabbit

Species Reactivity

Human, Rat

Immunogen

Recombinant Human Eukaryotic translation initiation factor 3 subunit I protein (1-323AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The EIF3I Antibody (Product code: CSB-PA03105A0Rb) is Non-conjugated. For EIF3I Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA03105B0Rb	EIF3I Antibody, HRP conjugated	ELISA
FITC	CSB-PA03105C0Rb	EIF3I Antibody, FITC conjugated
Biotin	CSB-PA03105D0Rb	EIF3I Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:2000-1:10000
IF	1:200-1:500

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.

Gene References into Functions

EIF3i may affect vesicular stomatitis virus (VSV) growth by regulating the host antiviral response in HeLa cells. PMID: 29173589
High eIF3i expression is associated with melanoma. PMID: 28193911
Clusterin is an independent predictive factor for prognosis of hepatocellular carcinoma and it facilitates metastasis through EIF3I/Akt/MMP13 signaling. PMID: 25609201
eIF3i is a proto-oncogene that drives colon oncogenesis by translationally upregulating COX-2 and activating the beta-catenin signaling pathway PMID: 24056964
findings report that short hairpin RNA mediated depletion of TRIP-1 gene transcripts in A549 cells promotes epithelial-mesenchymal transition (EMT); knockdown of TRIP-1 dramatically increased A549 responsiveness to TGF-beta1 induction of EMT PMID: 21378021
eIF3i overexpression fosters the integration of growth signals by mTOR into the mRNA translation process, promoting protein synthesis and tumor growth. PMID: 16929481
Subunit b of the eIF3 complex directly binds to HCV IRES domain III via its N-terminal-RRM. PMID: 19059401
TGF-beta receptor interacting protein 1 (TRIP-1), which we show inhibits fibroblast collagen contraction, is higher in fetal than adult fibroblasts PMID: 19329541

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Subcellular Location

Cytoplasm.

Protein Families

EIF-3 subunit I family

Database Links

HGNC: 3272

OMIM: 603911

KEGG: hsa:8668

STRING: 9606.ENSP00000362688

UniGene: Hs.530096

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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