FBXO22 Antibody

Datasheet

Code	CSB-PA854115ESR1HU
Size	US$166
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Image	Western Blot Positive WB detected in:Mouse Brain tissue lysate(20µg) All lanes: FBXO22 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/20000 dilution Predicted band size: 45kDa Observed band size: 45 kDa Exposure time: 10s Western Blot Positive WB detected in: Hela whole cell lysate(20µg), MCF7 whole cell lysate(20µg), HepG2 whole cell lysate(20µg), 293 whole cell lysate(20µg), JK whole cell lysate(20µg), K562 whole cell lysate(20µg), A549 whole cell lysate(20µg), A431 whole cell lysate(20µg), U251 whole cell lysate(20µg) All lanes: FBXO22 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/20000 dilution Predicted band size: 45 kDa Observed band size: 45 kDa Exposure time: 10s IHC image of CSB-PA854115ESR1HU diluted at 1:200 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody IHC image of CSB-PA854115ESR1HU diluted at 1:200 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) FBXO22 Polyclonal antibody

Uniprot No.

Q8NEZ5

Target Names

FBXO22

Alternative Names

0610033L19Rik antibody; 1600016C16Rik antibody; F box only protein 22 antibody; F box protein 22 antibody; F box protein FBX22p44 antibody; F-box only protein 22 antibody; F-box protein FBX22p44 antibody; FBX22 antibody; FBX22_HUMAN antibody; FBXO 22 antibody; FBXO22 antibody; FIST domain containing 1 antibody; FISTC1 antibody; FLJ13986 antibody; MGC124731 antibody; MGC31799 antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Synthesized peptide derived from human FBX22 AA range: 182-232

Immunogen Species

Homo sapiens (Human)

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:3000
IHC	1:100-1:300

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. Promotes the proteasome-dependent degradation of key sarcomeric proteins, such as alpha-actinin (ACTN2) and filamin-C (FLNC), essential for maintenance of normal contractile function.

Gene References into Functions

Data show that FBXO22 mediated the polyubiquitination and degradation of CD147 by interacting with CD147 intracellular domain (CD147-ICD). PMID: 28117675
Authors demonstrate that F-box only protein 22 (FBXO22) interacts with and thereby destabilizes KLF4 via polyubiquitination. As a result, FBXO22 could promote HCC cells proliferation. PMID: 26087183
Results indicate that SCF(Fbxo22)-KDM4A is an E3 ubiquitin ligase that targets methylated p53 and regulates key senescent processes. PMID: 26868148
FBXO22 protein is required for optimal synthesis of NMDA receptor coagonist D-serine by interacting with serine racemase, activating it, and preventing its targeting to membranes. PMID: 25336657
we determined that the phage-encoded GogB effector protein in Salmonella targets the host SCF E3 type ubiquitin ligase through an interaction with Skp1 and the human F-box only 22 (FBXO22) protein PMID: 22761574
FBXO22 is a key regulator of histone methylation marks, namely, H3K9 and H3K36 methylation, through the regulation of KDM4A protein levels. PMID: 21768309

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Subcellular Location

Cytoplasm, myofibril, sarcomere, Z line.

Tissue Specificity

Predominantly expressed in liver, also enriched in cardiac muscle.

Database Links

HGNC: 13593

OMIM: 609096

KEGG: hsa:26263

STRING: 9606.ENSP00000307833

UniGene: Hs.591115

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Protein Service

Gene Synthesis Service

Oligo Synthesis Service

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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