LPCAT2 Antibody

Datasheet

Code	CSB-PA013053LA01HU
Size	US$166
	Order now
Image	Western Blot Positive WB detected in: 293 whole cell lysate All lanes: LPCAT2 antibody at 5μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 61, 31 kDa Observed band size: 61 kDa IHC image of CSB-PA013053LA01HU diluted at 1:200 and staining in paraffin-embedded human thyroid tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA013053LA01HU diluted at 1:200 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
The Latest Promotion
Have Questions?	Leave a Message or Start an on-line Chat

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) LPCAT2 Polyclonal antibody

Uniprot No.

Q7L5N7

Target Names

LPCAT2

Alternative Names

1 acylglycerophosphocholine O acyltransferase antibody; 1 alkylglycerophosphocholine O acetyltransferase antibody; 1-acylglycerophosphocholine O-acyltransferase antibody; 1-alkylglycerophosphocholine O-acetyltransferase antibody; A330042H22 antibody; Acetyl CoA:lyso PAF acetyltransferase antibody; Acetyl-CoA:lyso-PAF acetyltransferase antibody; Acetyl-CoA:lyso-platelet-activating factor acetyltransferase antibody; acyl CoA:lysophosphatidylcholine acyltransferase 2 antibody; Acyl-CoA:lysophosphatidylcholine acyltransferase member 1 antibody; Acyltransferase like 1 antibody; Acyltransferase like 1A antibody; Acyltransferase-like 1 antibody; AYTL1 antibody; Aytl1a antibody; DKFZp686H22112 antibody; FLJ20481 antibody; lpafat1 antibody; LPC acyltransferase 2 antibody; LPCAT-2 antibody; LPCAT2 antibody; Lyso PAF acetyltransferase antibody; Lyso platelet activating factor (PAF) acetyltransferase antibody; Lyso-PAF acetyltransferase antibody; LysoPAFAT antibody; LysoPAFAT/LPCAT2 antibody; LysoPC acyltransferase 2 antibody; Lysophosphatidylcholine acyltransferase 2 antibody; OTTMUSP00000029641 antibody; PCAT2_HUMAN antibody; RGD1563994 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Lysophosphatidylcholine acyltransferase 2 protein (442-544AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The LPCAT2 Antibody (Product code: CSB-PA013053LA01HU) is Non-conjugated. For LPCAT2 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA013053LB01HU	LPCAT2 Antibody, HRP conjugated	ELISA
FITC	CSB-PA013053LC01HU	LPCAT2 Antibody, FITC conjugated
Biotin	CSB-PA013053LD01HU	LPCAT2 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

■ Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function

Exhibits both acyltransferase and acetyltransferase activities. Catalyzes the conversion of lysophosphatidylcholine (1-acyl-sn-glycero-3-phosphocholine or LPC) into phosphatidylcholine (1,2-diacyl-sn-glycero-3-phosphocholine or PC). Catalyzes the conversion 1-acyl-sn-glycerol-3-phosphate (lysophosphatidic acid or LPA) into 1,2-diacyl-sn-glycerol-3-phosphate (phosphatidic acid or PA) by incorporating an acyl moiety at the sn-2 position of the glycerol backbone. Involved in platelet-activating factor (PAF) biosynthesis by catalyzing the conversion of the PAF precursor, 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF) into 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (PAF). Also converts lyso-PAF to 1-O-alkyl-2-acyl-sn-glycero-3-phosphocholine (PC), a major component of cell membranes and a PAF precursor. Under resting conditions, acyltransferase activity is preferred. Upon acute inflammatory stimulus, acetyltransferase activity is enhanced and PAF synthesis increases. Involved in the regulation of lipid droplet number and size.

Gene References into Functions

three of these five genes (CXCL14, ITGAX, and LPCAT2) harbored polymorphisms associated with aggressive disease development in a human GWAS cohort consisting of 1,172 prostate cancer patients. PMID: 25411967
Data indicate a strong negative correlation between ALOX15, FADS2, and IL5RA expression with 2-arachidonoylglycerophosphocholine levels in dual asthmatic responses. PMID: 23844124
LPCAT1 and -2 have, in addition to their known function in specialized cells, a ubiquitous role in LD-associated lipid metabolism PMID: 21498505
Our enzymatic assays strongly suggest that the cDNA previously identified as LPCAT2/lyso platelet-activating factor-acetyltransferase cDNA has AGPAT activity and thus we prefer to identify this clone as AGPAT11 as well. PMID: 20363836
The enzyme acetyl-CoA:LYSO-PAF acetyltransferase catalyzes not only biosynthesis of PAF upon acute inflammatory stimulation but also incorporation of arachidonoyl-CoA to produce PAF precursor membrane glycerophospholipids for membrane biogenesis. PMID: 17182612

Hide All

Subcellular Location

Endoplasmic reticulum membrane; Single-pass type II membrane protein. Golgi apparatus membrane; Single-pass type II membrane protein. Cell membrane; Single-pass type II membrane protein. Lipid droplet.

Protein Families

1-acyl-sn-glycerol-3-phosphate acyltransferase family

Database Links

HGNC: 26032

OMIM: 612040

KEGG: hsa:54947

STRING: 9606.ENSP00000262134

UniGene: Hs.460857

Promotions

Transmembrane Protein Questionnaire with Gifts

Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

Join us with

Subscribe newsletter

Leave a message

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：

Place an order now

I. Product details

Product name *

Code *

Size *

Quantity *

Message

II. Contact details

Name *

Email address *

Company/Organization

Phone

III. Ship To

Address *

City *

State/Province *

Zip/Post code *

Country *

Attn Name *

Phone# *

IV. Bill To Same as Shipping Address