MAP3K9 Antibody

Datasheet

Code	CSB-PA013434LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Rat lung tissue All lanes: MAP3K9 antibody at 2.7μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 122, 124 kDa Observed band size: 122 kDa IHC image of CSB-PA013434LA01HU diluted at 1:400 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of A549 cells with CSB-PA013434LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) MAP3K9 Polyclonal antibody

Uniprot No.

P80192

Target Names

MAP3K9

Alternative Names

M3K9_HUMAN antibody; Map3k9 antibody; MEKK9 antibody; Mitogen activated protein kinase kinase kinase 9 antibody; Mitogen-activated protein kinase kinase kinase 9 antibody; Mixed lineage kinase 1 (tyr and ser/thr specificity) antibody; Mixed lineage kinase 1 antibody; MLK1 antibody; PRKE1 antibody

Raised in

Rabbit

Species Reactivity

Human, Rat

Immunogen

Recombinant Human Mitogen-activated protein kinase kinase kinase 9 protein (864-1098AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The MAP3K9 Antibody (Product code: CSB-PA013434LA01HU) is Non-conjugated. For MAP3K9 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA013434LB01HU	MAP3K9 Antibody, HRP conjugated	ELISA
FITC	CSB-PA013434LC01HU	MAP3K9 Antibody, FITC conjugated
Biotin	CSB-PA013434LD01HU	MAP3K9 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:100-1:500
IF	1:50-1:500

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade through the phosphorylation of MAP2K4/MKK4 and MAP2K7/MKK7 which in turn activate the JNKs. The MKK/JNK signaling pathway regulates stress response via activator protein-1 (JUN) and GATA4 transcription factors. Plays also a role in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis.

Gene References into Functions

Data report that MKL1 was both sufficient and necessary for p65-dependent pro-inflammatory transcriptional program. MKL1 deficiency erased key histone modifications synonymous with transactivation on p65 target promoters. Specifically, MKL1 defined histone H3K4 trimethylation landscape for NF-kappaB dependent transcription. PMID: 28298643
There was a negatively relationship between miR-15a and MAP3K9 expression in NP cells. PMID: 28081468
miR148b directly targeted mitogenactivated protein kinase (MAPK) kinase kinase 9 (MAP3K9), an upstream activator of MAPK kinase/cJun Nterminal kinase (JNK) signaling, suppressing the protein but not the mRNA levels. PMID: 26573018
identified three kinases with gain-of-function mutations in lung cancer, namely FGFR4, MAP3K9, and PAK5. Mutations in these kinases are activating toward the ERK pathway, and targeted depletion of the mutated kinases inhibits proliferation PMID: 23836671
estrogen receptor-alpha transcriptional activity is repressed by the Rho/megakaryoblastic leukemia 1 signaling pathway PMID: 19826002

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Involvement in disease

May play a role in esophageal cancer susceptibility and/or development.

Protein Families

Protein kinase superfamily, STE Ser/Thr protein kinase family, MAP kinase kinase kinase subfamily

Tissue Specificity

Expressed in epithelial tumor cell lines of colonic, breast and esophageal origin.

Database Links

HGNC: 6861

OMIM: 600136

KEGG: hsa:4293

STRING: 9606.ENSP00000451263

UniGene: Hs.445496

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Protein Service

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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