MED30 Antibody

Code CSB-PA822225LA01HU
Size US$166
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  • Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA822225LA01HU at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human pancreatic tissue using CSB-PA822225LA01HU at dilution of 1:100

  • Immunofluorescent analysis of PC-3 cells using CSB-PA822225LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) MED30 Polyclonal antibody
Uniprot No.
Target Names
MED30
Alternative Names
MED30 antibody; MED30_HUMAN antibody; Mediator complex subunit 30 antibody; Mediator of RNA polymerase II transcription subunit 30 antibody; THRAP6 antibody; Thyroid hormone receptor-associated protein 6 antibody; Thyroid hormone receptor-associated protein complex 25 kDa component antibody; TRAP/Mediator complex component TRAP25 antibody; Trap25 antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human Mediator of RNA polymerase II transcription subunit 30 protein (67-165AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The MED30 Antibody (Product code: CSB-PA822225LA01HU) is Non-conjugated. For MED30 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA822225LB01HU MED30 Antibody, HRP conjugated ELISA
FITC CSB-PA822225LC01HU MED30 Antibody, FITC conjugated
Biotin CSB-PA822225LD01HU MED30 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, IHC, IF
Recommended Dilution
Application Recommended Dilution
IHC 1:20-1:200
IF 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Gene References into Functions
  1. MED30 seems to be involved in the progression of the renal cell carcinoma (RCC). PMID: 29661722
  2. Knockdown of MED30 led to reduction of the tumour parameters proliferation, migration, and invasion in the bladder cancer (BCa) cell lines. PMID: 29187445
  3. analysis of a novel transcript of MED30, termed MED30 short (MED30s) generated by alternative splicing PMID: 20493979
  4. Requirement of TRAP/mediator for both activator-independent and activator-dependent transcription in conjunction with TFIID-associated TAF(II)s. PMID: 11909976
Subcellular Location
Nucleus.
Protein Families
Mediator complex subunit 30 family
Tissue Specificity
Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle.
Database Links

HGNC: 23032

OMIM: 610237

KEGG: hsa:90390

STRING: 9606.ENSP00000297347

UniGene: Hs.492612

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