MIS12 Antibody

Code CSB-PA875639LA01HU
Size US$166
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  • Western blot
    All lanes: MIS12 antibody at 2μg/ml + Hela whole cell lysate
    Secondary
    Goat polyclonal to rabbit IgG at 1/10000 dilution
    Predicted band size: 25 kDa
    Observed band size: 25 kDa

  • Immunohistochemistry of paraffin-embedded human gastric cancer using CSB-PA875639LA01HU at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA875639LA01HU at dilution of 1:100

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) MIS12 Polyclonal antibody
Uniprot No.
Target Names
MIS12
Alternative Names
MIS12 antibody; Protein MIS12 homolog antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human Protein MIS12 homolog protein (1-305AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The MIS12 Antibody (Product code: CSB-PA875639LA01HU) is Non-conjugated. For MIS12 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA875639LB01HU MIS12 Antibody, HRP conjugated ELISA
FITC CSB-PA875639LC01HU MIS12 Antibody, FITC conjugated
Biotin CSB-PA875639LD01HU MIS12 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
IHC 1:20-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Part of the MIS12 complex which is required for normal chromosome alignment and segregation and for kinetochore formation during mitosis. Essential for proper kinetochore microtubule attachments.
Gene References into Functions
  1. Whereas CENP-C recruits a single MIS12:NDC80 complex, the authors show here that CENP-T binds one MIS12:NDC80 and two NDC80 complexes upon phosphorylation by the mitotic CDK1:Cyclin B complex at three distinct CENP-T sites. PMID: 28012276
  2. RWD-containing proteins interact with the Mis12 complex to shape KMN network topology, as revealed by structural analysis PMID: 24530301
  3. Direct binding of Cenp-C to the Mis12 complex joins the inner and outer kinetochore. PMID: 21353556
  4. These findings support a novel role for Hsp90-Sgt1 chaperones in ensuring the fidelity of Mis12 multiprotein complex assembly. PMID: 20404110
  5. hMIS12 has a role in mitosis and localizes at kinetochores PMID: 12515822
  6. These results indicate that a four-subunit Mis12 complex plays an essential role in chromosome segregation in vertebrates and contributes to mitotic kinetochore assembly. PMID: 16585270

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Subcellular Location
Chromosome, centromere, kinetochore.
Protein Families
Mis12 family
Database Links

HGNC: 24967

OMIM: 609178

KEGG: hsa:79003

STRING: 9606.ENSP00000370557

UniGene: Hs.267194

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