MTRF1 Antibody

Code CSB-PA015197LA01HU
Size US$166
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Image
  • Immunofluorescence staining of Hela cells with CSB-PA015197LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA015197LA01HU at dilution of 1:100

  • Western Blot
    Positive WB detected in: Hela whole cell lysate
    All lanes: MTRF1 antibody at 3μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 53, 55 kDa
    Observed band size: 53 kDa

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) MTRF1 Polyclonal antibody
Uniprot No.
Target Names
MTRF1
Alternative Names
mitochondrial antibody; MRF-1 antibody; MRF1 antibody; MtRF-1 antibody; MTRF1 antibody; Peptide chain release factor 1 antibody; Peptide chain release factor 1; mitochondrial antibody; RF1M_HUMAN antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human Peptide chain release factor 1, mitochondrial protein (285-433AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The MTRF1 Antibody (Product code: CSB-PA015197LA01HU) is Non-conjugated. For MTRF1 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA015197LB01HU MTRF1 Antibody, HRP conjugated ELISA
FITC CSB-PA015197LC01HU MTRF1 Antibody, FITC conjugated
Biotin CSB-PA015197LD01HU MTRF1 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:20-1:200
IF 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Mitochondrial peptide chain release factor that directs the termination of translation in response to the peptide chain non-cognate termination stop codons AGG and AGA.
Gene References into Functions
  1. Purified mtRF1 did not induce translation termination in vitro nor did mtRF1 restore mitochondrial translation machinery in mutated S. cerevisiae in vivo. A different mtRF, mtRF1a, was found. PMID: 17803939
Subcellular Location
Mitochondrion.
Protein Families
Prokaryotic/mitochondrial release factor family
Database Links

HGNC: 7469

OMIM: 604601

KEGG: hsa:9617

STRING: 9606.ENSP00000368790

UniGene: Hs.382176

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