MYRF Antibody

Datasheet

Code	CSB-PA897527LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Mouse brain tissue All lanes: MYRF antibody at 3.2μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 125, 121 kDa Observed band size: 125 kDa Immunohistochemistry of paraffin-embedded human lung tissue using CSB-PA897527LA01HU at dilution of 1:100 Immunofluorescence staining of A549 cells with CSB-PA897527LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) MYRF Polyclonal antibody

Uniprot No.

Q9Y2G1

Target Names

MYRF

Alternative Names

C11orf9 antibody; Chromosome 11 open reading frame 9 antibody; GM1804 antibody; GM98 antibody; KIAA0954 antibody; MGC10781 antibody; MRF antibody; MRF_HUMAN antibody; Myelin gene regulatory factor antibody; Ndt80 antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human Myelin regulatory factor protein (843-1004AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The MYRF Antibody (Product code: CSB-PA897527LA01HU) is Non-conjugated. For MYRF Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA897527LB01HU	MYRF Antibody, HRP conjugated	ELISA
FITC	CSB-PA897527LC01HU	MYRF Antibody, FITC conjugated
Biotin	CSB-PA897527LD01HU	MYRF Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:200
IF	1:20-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The peptide mapping within the amino acids 843-1004 of the recombinant human MYRF protein is used to immunize rabbits to get rabbit antiserum. The rabbit antiserum is further purified by protein G to get the anti-MYRF antibody. It is a polyclonal antibody and occurs as an unconjugated IgG. Its purity is greater than 95%. It is reactive with human and mouse MYRF protein, a membrane-bound transcription factor required for oligodendrocyte (OL) differentiation and myelin formation ＆ maintenance. And this MYRF antibody is suitable for the detection of the MYRF protein in ELISA, WB, and IHC application.

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Target Background

Function

Constitutes a precursor of the transcription factor. Mediates the autocatalytic cleavage that releases the Myelin regulatory factor, N-terminal component that specifically activates transcription of central nervous system (CNS) myelin genes.; Membrane-bound part that has no transcription factor activity and remains attached to the endoplasmic reticulum membrane following cleavage.; Transcription factor that specifically activates expression of myelin genes such as MBP, MOG, MAG, DUSP15 and PLP1 during oligodendrocyte (OL) maturation, thereby playing a central role in oligodendrocyte maturation and CNS myelination. Specifically recognizes and binds DNA sequence 5'-CTGGYAC-3' in the regulatory regions of myelin-specific genes and directly activates their expression. Not only required during oligodendrocyte differentiation but is also required on an ongoing basis for the maintenance of expression of myelin genes and for the maintenance of a mature, viable oligodendrocyte phenotype.

Gene References into Functions

The relative scarcity of oligodendrocyte lineage cells expressing MYRF in demyelinated MS lesions demonstrates, for the first time, that chronic lesions lack oligodendrocytes that express this necessary transcription factor for remyelination and supports the notion that a failure to fully differentiate underlies remyelination failure. PMID: 28631093
molecular mechanisms underlying the homo-trimerization of Myrf N-terminal fragments and the homo-trimeric DNA binding of Myrf N-terminal fragments PMID: 28160598
A Bacteriophage tailspike domain promotes self-cleavage of a human membrane-bound transcription factor, the myelin regulatory factor MYRF. PMID: 23966832

Subcellular Location

[Myelin regulatory factor]: Endoplasmic reticulum membrane; Single-pass membrane protein.; [Myelin regulatory factor, N-terminal]: Nucleus. Cytoplasm.; [Myelin regulatory factor, C-terminal]: Endoplasmic reticulum membrane; Single-pass membrane protein.

Protein Families

MRF family

Tissue Specificity

Expressed in lung, ARPE-19 cell line, brainstem, uterus and, to a lesser extent, in basal ganglion and liver. Weakly expressed in cerebellum and retina.

Database Links

HGNC: 1181

OMIM: 608329

KEGG: hsa:745

STRING: 9606.ENSP00000278836

UniGene: Hs.473109

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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