CIAO3 Antibody

Code CSB-PA863991LA01HU
Size US$166
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  • Western Blot
    Positive WB detected in: A549 whole cell lysate
    All lanes: CIAO3 antibody at 2.7µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 54, 27, 43 kDa
    Observed band size: 54, 27 kDa

  • Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA863991LA01HU at dilution of 1:100

  • Immunofluorescent analysis of U251 cells using CSB-PA863991LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) CIAO3 Polyclonal antibody
Uniprot No.
Target Names
CIAO3
Alternative Names
CIAO3 antibody; NARFL antibody; PRNCytosolic iron-sulfur assembly component 3 antibody; Cytosolic Fe-S cluster assembly factor NARFL antibody; Iron-only hydrogenase-like protein 1 antibody; IOP1 antibody; Nuclear prelamin A recognition factor-like protein antibody; Protein related to Narf antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human Cytosolic iron-sulfur assembly component 3 protein (2-265AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The CIAO3 Antibody (Product code: CSB-PA863991LA01HU) is Non-conjugated. For CIAO3 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA863991LB01HU CIAO3 Antibody, HRP conjugated ELISA
FITC CSB-PA863991LC01HU CIAO3 Antibody, FITC conjugated
Biotin CSB-PA863991LD01HU CIAO3 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:20-1:200
IF 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function
Component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into extramitochondrial Fe/S proteins. Seems to negatively regulate the level of HIF1A expression, although this effect could be indirect.
Gene References into Functions
  1. we concluded that the Ser161Ile mutant induced NARFL deficiency and eventually diffuse PAVMs probably through VEGF pathway. In a word, we detected a functional mutation in NARFL, which might be the pathogenic gene in this pedigree. PMID: 27835862
  2. IOP1 is involved in mammalian cytosolic Fe-S protein maturation. PMID: 18270200
  3. human IscA1 plays an important role in both mitochondrial and cytosolic iron-sulfur cluster biogenesis, and a notable component of the latter is the interaction between IscA1 and IOP1. PMID: 19864422
Protein Families
NARF family
Tissue Specificity
Widely expressed.
Database Links

HGNC: 14179

OMIM: 611118

KEGG: hsa:64428

STRING: 9606.ENSP00000251588

UniGene: Hs.513247

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