NEK3 Antibody

Datasheet

Code	CSB-PA015701ESR1HU
Size	US$166
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Image	Western blot All lanes: NEK3 antibody at 6.04µg/ml Lane 1: MCF-7 whole cell lysate Lane 2: Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 58, 56 kDa Observed band size: 58 kDa Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA015701ESR1HU at dilution of 1:100 Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA015701ESR1HU at dilution of 1:100
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) NEK3 Polyclonal antibody

Uniprot No.

P51956

Target Names

NEK3

Alternative Names

NEK3 antibody; Serine/threonine-protein kinase Nek3 antibody; EC 2.7.11.1 antibody; HSPK 36 antibody; Never in mitosis A-related kinase 3 antibody; NimA-related protein kinase 3 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Serine/threonine-protein kinase Nek3 protein (267-506AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:20-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Protein kinase which influences neuronal morphogenesis and polarity through effects on microtubules. Regulates microtubule acetylation in neurons. Contributes to prolactin-mediated phosphorylation of PXN and VAV2. Implicated in prolactin-mediated cytoskeletal reorganization and motility of breast cancer cells through mechanisms involving RAC1 activation and phosphorylation of PXN and VAV2.

Gene References into Functions

NEK3 kinase phosphorylates SNAP29 on its serine 105. NEK3-mediated phosphorylation determines membrane localization of SNAP29. Membrane-associated SNAP29 regulates Golgi and focal adhesion structures. PMID: 29454964
The data demonstrate that NEK3 is overexpressed in gastric cancer, which promotes the malignancy of gastric cancer PMID: 29504992
these data support a modulatory role for phosphorylation at NEK3 Thr-165 in focal adhesion maturation and/or turnover to promote breast cancer cell migration. PMID: 27489110
EHD2 interacts with Nek3 [NIMA (never in mitosis in Aspergillus nidulans)-related kinase 3], a serine/threonine kinase. PMID: 21756249
Molecular cloning and characterization of the human NIMA-related protein kinase 3 gene (NEK3). PMID: 12063396
Nek3 insertion/deletion polymorphism with alterations at 13q14 is associated with cancers PMID: 17118778
Nek3 contributes to prolactin-mediated breast cancer motility through mechanisms involving Rac1 activation and paxillin phosphorylation. PMID: 17297458

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Subcellular Location

Cytoplasm. Cell projection, axon.

Protein Families

Protein kinase superfamily, NEK Ser/Thr protein kinase family, NIMA subfamily

Tissue Specificity

Up-regulated in malignant versus normal breast tissue. Isoform 2 shows a high level of expression in testis, ovary and brain.

Database Links

HGNC: 7746

OMIM: 604044

KEGG: hsa:4752

STRING: 9606.ENSP00000383210

UniGene: Hs.409989

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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