NELFB Antibody

Datasheet

Code	CSB-PA03765A0Rb
Size	US$166
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Image	Western Blot All lanes: NELFB antibody at 2µg/ml Lane 1: NIH/3T3 whole cell lysate Lane 2: HL60 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 66 kDa Observed band size: 66 kDa Immunohistochemistry of paraffin-embedded human endometrial cancer using CSB-PA03765A0Rb at dilution of 1:100 Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA03765A0Rb at dilution of 1:100 Chromatin Immunoprecipitation Hela (1.1*10⁶) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4µg anti-NELFB or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers (CSB-PP03765HU) against the TFF1 promoter.
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) NELFB Polyclonal antibody

Uniprot No.

Q8WX92

Target Names

NELFB

Alternative Names

NELFB antibody; COBRA1 antibody; KIAA1182 antibody; Negative elongation factor B antibody; NELF-B antibody; Cofactor of BRCA1 antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human Negative elongation factor B protein (8-199AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The NELFB Antibody (Product code: CSB-PA03765A0Rb) is Non-conjugated. For NELFB Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA03765B0Rb	NELFB Antibody, HRP conjugated	ELISA
FITC	CSB-PA03765C0Rb	NELFB Antibody, FITC conjugated
Biotin	CSB-PA03765D0Rb	NELFB Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, ChIP

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:20-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Native Chromatin Immunoprecipitation(ChIP) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The NELFB antibody (CSB-PA03765A0Rb) is produced in the rabbit with a peptide corresponding to amino acids 8-199 of the human NELFB immunized. This polyclonal antibody occurs as an unconjugated IgG. Its purity is 95%+ using protein G purified. It is reactive with human and mouse samples. The NELFB protein is a subunit of NELF, which is known to enforce promoter-proximal pausing of RNA polymerase II. The functional cardiac deterioration in NELFB knockout mice strongly indicates an important role of NELFB in the maintenance of normal cardiac contractility under stressed conditions. This anti-NELFB antibody has been quality-verified in ELISA, WB, IHC, and ChIP applications.

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Target Background

Function

Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. May be able to induce chromatin unfolding. Essential for early embryogenesis; plays an important role in maintaining the undifferentiated state of embryonic stem cells (ESCs) by preventing unscheduled expression of developmental genes. Plays a key role in establishing the responsiveness of stem cells to developmental cues; facilitates plasticity and cell fate commitment in ESCs by establishing the appropriate expression level of signaling molecules. Supports the transcription of genes involved in energy metabolism in cardiomyocytes; facilitates the association of transcription initiation factors with the promoters of the metabolism-related genes.; (Microbial infection) The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II. In vitro, binds weakly to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1.

Gene References into Functions

BRCA1 Interacting Protein COBRA1 Facilitates Adaptation to Castrate-Resistant Growth Conditions. PMID: 30036938
A positively charged face of NELF-AC is involved in RNA binding, whereas the opposite face of the NELF-AC subcomplex binds NELF-B. NELF-B is predicted to form a HEAT repeat fold, also binds RNA in vivo, and anchors the subunit NELF-E, which is confirmed to bind RNA in vivo. PMID: 27282391
High COBRA1 expression is associated with hepatocellular carcinoma. PMID: 28112367
both human and mouse NELF-B proteins are translated from a non-AUG codon upstream of the annotated AUG. PMID: 26010750
These studies allow us to position the actions of two new modulators of GR-regulated transactivation, NELF-A and NELF-B, relative to other factors in the overall gene induction sequence. PMID: 24097989
data show that NELF subunits exhibit highly specific subcellular localizations, such as in NELF bodies or in midbodies, and some shuttle actively between the nucleus and cytoplasm; loss of NELF from cells can lead to enlarged and/or multiple nuclei PMID: 19245807
NELF-C and NELF-D are highly related or identical to the protein called TH1, of unknown function. NELF-B and NELF-C or NELF-D are integral subunits that bring NELF-A and NELF-E together. [NELF-B] [NELF-C] PMID: 12612062
differentially expressed in breast cancer cell lines, antibody to it may be a useful tool to investigate its functions PMID: 15185750
COBRA1 may directly modulate AP-1 pathway and, therefore, may play important roles in cell proliferation, differentiation, apoptosis, and oncogenesis. PMID: 15530430
COBRA1 can negatively regulate the activator protein-1 (AP-1) complex at the TFF1 promoter and thus down-regulate TFF1 expression in gastric cancer cell lines. PMID: 16452188
Decreasing NELF also correlated with displacement of a positioned nucleosome and increased acetylation of histone H4, suggesting coupling of transcription elongation and chromatin remodeling PMID: 17442680
Negative elongation factor (NELF) is a four subunit transcription factor. Our results point to a surprising role of NELF in the 3' end processing of histone mRNAs and suggest that NELF is a new factor that coordinates mRNA processing in transcription. PMID: 17499042
COBRA1 may coordinate multiple steps in ligand-dependent gene expression, which in turn ensures both the quantity and quality of hormone-stimulated gene products PMID: 17659869
a lack of COBRA1 expression in breast carcinoma may serve as a useful indicator for poor prognosis. PMID: 17910036
COBRA1 and BRCA1 may engage in common gene regulatory pathways to suppress breast cancer progression. PMID: 18071589

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Subcellular Location

Nucleus.

Protein Families

NELF-B family

Tissue Specificity

Widely expressed. Expressed in heart, brain, lung, placenta, liver, skeletal muscle, kidney and pancreas.

Database Links

HGNC: 24324

OMIM: 611180

KEGG: hsa:25920

STRING: 9606.ENSP00000339495

UniGene: Hs.521809

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