NSMCE2 Antibody

Datasheet

Code	CSB-PA836260LA01HU
Size	US$166
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Image	IHC image of CSB-PA836260LA01HU diluted at 1:600 and staining in paraffin-embedded human melanoma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) NSMCE2 Polyclonal antibody

Uniprot No.

Q96MF7

Target Names

NSMCE2

Alternative Names

C8orf36 antibody; E3 SUMO-protein ligase NSE2 antibody; hMMS21 antibody; hNSE2 antibody; methyl methanesulfonate sensitivity gene 21 antibody; MMS21 antibody; MMS21 homolog antibody; non-SMC element 2 antibody; Non-SMC element 2 homolog antibody; Non-structural maintenance of chromosomes element 2 homolog antibody; NSE2 antibody; NSE2_HUMAN antibody; Nsmce2 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human E3 SUMO-protein ligase NSE2 protein (1-247AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The NSMCE2 Antibody (Product code: CSB-PA836260LA01HU) is Non-conjugated. For NSMCE2 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA836260LB01HU	NSMCE2 Antibody, HRP conjugated	ELISA
FITC	CSB-PA836260LC01HU	NSMCE2 Antibody, FITC conjugated
Biotin	CSB-PA836260LD01HU	NSMCE2 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, IHC

Recommended Dilution

Application	Recommended Dilution
IHC	1:500-1:1000

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

E3 SUMO-protein ligase component of the SMC5-SMC6 complex, a complex involved in DNA double-strand break repair by homologous recombination. Is not be required for the stability of the complex. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs). Acts as an E3 ligase mediating SUMO attachment to various proteins such as SMC6L1 and TSNAX, the shelterin complex subunits TERF1, TERF2, TINF2 and TERF2IP, RAD51AP1, and maybe the cohesin components RAD21 and STAG2. Required for recruitment of telomeres to PML nuclear bodies. SUMO protein-ligase activity is required for the prevention of DNA damage-induced apoptosis by facilitating DNA repair, and for formation of APBs in ALT cell lines. Required for sister chromatid cohesion during prometaphase and mitotic progression.

Gene References into Functions

Absence of NSMCE2 affects survival upon topoisomerase II inhibitor etoposide-induced DNA double-strand breaks double-strand breaks. PMID: 27792189
our study identified novel PVT1-NSMCE2 and CCDC26-NSMCE2 fusion genes that may play functional roles in leukemia. PMID: 25245984
support a role for NSMCE2 in recovery from DNA damage and raise the possibility that loss of its function produces dwarfism through reduced tolerance of replicative stress PMID: 25105364
show that the Smc5/6 subunit Mms21 sumoylates multiple lysines of the cohesin subunit Scc1 PMID: 22751501
Studies indicate that Nse1 may function as a ubiquitin ligase, and is targeted to chromatin through its interaction with the Smc5/6 complex. PMID: 21550342
Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator) PMID: 20379614
Observational study of gene-disease association. (HuGE Navigator) PMID: 20460480
the human SMC5/6 complex and the SUMO ligase activity of hMMS21 are required for the prevention of DNA damage-induced apoptosis by facilitating DNA repair in human cells PMID: 16055714
MMS21 dependent sumoylation is integral and important to the cohesion mechanism and mitotic progression and that this function appears to be independent of SMC6. PMID: 19502785
Observational study of gene-disease association. (HuGE Navigator) PMID: 19549807
Observational study of gene-disease association. (HuGE Navigator) PMID: 18163389

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Involvement in disease

Seckel syndrome 10 (SCKL10)

Subcellular Location

Nucleus. Chromosome, telomere. Nucleus, PML body.

Protein Families

NSE2 family

Database Links

HGNC: 26513

OMIM: 617246

KEGG: hsa:286053

STRING: 9606.ENSP00000287437

UniGene: Hs.388297

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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