PPM1F Antibody

Code CSB-PA018493LA01HU
Size US$166
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Image
  • Western Blot
    Positive WB detected in: Mouse liver tissue
    All lanes: PPM1F antibody at 3μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 50, 39 kDa
    Observed band size: 50 kDa

  • IHC image of CSB-PA018493LA01HU diluted at 1:100 and staining in paraffin-embedded human melanoma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • IHC image of CSB-PA018493LA01HU diluted at 1:100 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of HepG2 cells with CSB-PA018493LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) PPM1F Polyclonal antibody
Uniprot No.
Target Names
PPM1F
Alternative Names
Ca(2+)/calmodulin-dependent protein kinase phosphatase antibody; CaM-kinase phosphatase antibody; CAMKP antibody; CaMKPase antibody; FEM 2 antibody; hFEM-2 antibody; KIAA0015 antibody; Partner of PIX 2 antibody; POPX2 antibody; PP2C phosphatase antibody; Ppm1f antibody; PPM1F_HUMAN antibody; Protein fem-2 homolog antibody; Protein phosphatase 1F (PP2C domain containing) antibody; Protein phosphatase 1F antibody; Protein phosphatase Mg2+/Mn2+ dependent 1F antibody
Raised in
Rabbit
Species Reactivity
Human, Mouse
Immunogen
Recombinant Human Protein phosphatase 1F protein (1-76AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The PPM1F Antibody (Product code: CSB-PA018493LA01HU) is Non-conjugated. For PPM1F Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA018493LB01HU PPM1F Antibody, HRP conjugated ELISA
FITC CSB-PA018493LC01HU PPM1F Antibody, FITC conjugated
Biotin CSB-PA018493LD01HU PPM1F Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:20-1:200
IF 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Dephosphorylates and concomitantly deactivates CaM-kinase II activated upon autophosphorylation, and CaM-kinases IV and I activated upon phosphorylation by CaM-kinase kinase. Promotes apoptosis.
Gene References into Functions
  1. PPM1F overexpression suppressed cell proliferation and invasion and counteracted the tumour-promoting role of miR-590, but PPM1F knockdown reversed these effects. PMID: 29473240
  2. POPX2 is a negative regulator of TAK1 signaling pathway and modulates apoptosis through the regulation of TAK1 activity. PMID: 28906490
  3. PPM1F could work downstream of alpha9-nAchR to promote nicotine-induced carcinogenic signals. Thus, PPM1F expression could be used for prognostic diagnosis, or inhibited as a potential strategy for cancer prevention and therapy. PMID: 27769050
  4. This study, combined with our previous findings, suggests that a single ubiquitously expressed phosphatase POPX2 influences cancer metastasis via modulating multiple biological processes including MAPK signaling and exosome cytokine secretion. PMID: 27976581
  5. Overexpression of PPM1F is associated with metastasis of hepatocellular carcinoma. PMID: 26498692
  6. POPX2 affects trafficking by determining the phosphorylation status of KIF3A at serine 690. PMID: 24338362
  7. POPX2 might regulate cell motility through its regulation of the MAPK1/3, leading to changes in the cytoskeleton and cell motility. PMID: 23621870
  8. hCaMKP activity is reversibly regulated by oxidation/reduction at Cys-359 PMID: 22743349
  9. FHOD1 and PPM1F (direct regulators of the actin cytoskeleton) were identified as novel targets of miR-200c. Expression levels of FHOD1 and PPM1F were inversely correlated with miR-200c level in breast cancer cell lines and breast cancer patient samples. PMID: 22144583
  10. Data identify a biochemical pathway through which POPX2 exerts its apparent cellular function: the regulation of activity of glycogen synthase kinase-3, which in turn modulates extracellular signal-regulated kinase and cell motility. PMID: 21656682
  11. The POPX2 phosphatase regulates cancer cell motility and invasiveness. PMID: 20016286
  12. The p21-activated kinase PAK is negatively regulated by POPX1 and POPX2, a pair of serine/threonine phosphatases of the PP2C family. (POPX1; POPX2) PMID: 11864573
  13. PPM1F plays a key role in the regulation of CaMKII intracellularly PMID: 15140879

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Protein Families
PP2C family
Database Links

HGNC: 19388

KEGG: hsa:9647

STRING: 9606.ENSP00000263212

UniGene: Hs.112728

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