PPM1F Antibody

Datasheet

Code	CSB-PA018493LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Mouse liver tissue All lanes: PPM1F antibody at 3μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 50, 39 kDa Observed band size: 50 kDa IHC image of CSB-PA018493LA01HU diluted at 1:100 and staining in paraffin-embedded human melanoma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA018493LA01HU diluted at 1:100 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of HepG2 cells with CSB-PA018493LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) PPM1F Polyclonal antibody

Uniprot No.

P49593

Target Names

PPM1F

Alternative Names

Ca(2+)/calmodulin-dependent protein kinase phosphatase antibody; CaM-kinase phosphatase antibody; CAMKP antibody; CaMKPase antibody; FEM 2 antibody; hFEM-2 antibody; KIAA0015 antibody; Partner of PIX 2 antibody; POPX2 antibody; PP2C phosphatase antibody; Ppm1f antibody; PPM1F_HUMAN antibody; Protein fem-2 homolog antibody; Protein phosphatase 1F (PP2C domain containing) antibody; Protein phosphatase 1F antibody; Protein phosphatase Mg2+/Mn2+ dependent 1F antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human Protein phosphatase 1F protein (1-76AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The PPM1F Antibody (Product code: CSB-PA018493LA01HU) is Non-conjugated. For PPM1F Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA018493LB01HU	PPM1F Antibody, HRP conjugated	ELISA
FITC	CSB-PA018493LC01HU	PPM1F Antibody, FITC conjugated
Biotin	CSB-PA018493LD01HU	PPM1F Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Dephosphorylates and concomitantly deactivates CaM-kinase II activated upon autophosphorylation, and CaM-kinases IV and I activated upon phosphorylation by CaM-kinase kinase. Promotes apoptosis.

Gene References into Functions

PPM1F overexpression suppressed cell proliferation and invasion and counteracted the tumour-promoting role of miR-590, but PPM1F knockdown reversed these effects. PMID: 29473240
POPX2 is a negative regulator of TAK1 signaling pathway and modulates apoptosis through the regulation of TAK1 activity. PMID: 28906490
PPM1F could work downstream of alpha9-nAchR to promote nicotine-induced carcinogenic signals. Thus, PPM1F expression could be used for prognostic diagnosis, or inhibited as a potential strategy for cancer prevention and therapy. PMID: 27769050
This study, combined with our previous findings, suggests that a single ubiquitously expressed phosphatase POPX2 influences cancer metastasis via modulating multiple biological processes including MAPK signaling and exosome cytokine secretion. PMID: 27976581
Overexpression of PPM1F is associated with metastasis of hepatocellular carcinoma. PMID: 26498692
POPX2 affects trafficking by determining the phosphorylation status of KIF3A at serine 690. PMID: 24338362
POPX2 might regulate cell motility through its regulation of the MAPK1/3, leading to changes in the cytoskeleton and cell motility. PMID: 23621870
hCaMKP activity is reversibly regulated by oxidation/reduction at Cys-359 PMID: 22743349
FHOD1 and PPM1F (direct regulators of the actin cytoskeleton) were identified as novel targets of miR-200c. Expression levels of FHOD1 and PPM1F were inversely correlated with miR-200c level in breast cancer cell lines and breast cancer patient samples. PMID: 22144583
Data identify a biochemical pathway through which POPX2 exerts its apparent cellular function: the regulation of activity of glycogen synthase kinase-3, which in turn modulates extracellular signal-regulated kinase and cell motility. PMID: 21656682
The POPX2 phosphatase regulates cancer cell motility and invasiveness. PMID: 20016286
The p21-activated kinase PAK is negatively regulated by POPX1 and POPX2, a pair of serine/threonine phosphatases of the PP2C family. (POPX1; POPX2) PMID: 11864573
PPM1F plays a key role in the regulation of CaMKII intracellularly PMID: 15140879

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Protein Families

PP2C family

Database Links

HGNC: 19388

KEGG: hsa:9647

STRING: 9606.ENSP00000263212

UniGene: Hs.112728

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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