RNF144B Antibody

Datasheet

Code	CSB-PA253689
Size	US$166
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Image	The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using CSB-PA253689(RNF144B Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using CSB-PA253689(RNF144B Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200) Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: TM4 cells, Primary antibody: CSB-PA253689(RNF144B Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes
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Product Details
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Target Background

Product Details

Uniprot No.

Q7Z419

Target Names

RNF144B

Alternative Names

RNF144B; IBRDC2; P53RFP; E3 ubiquitin-protein ligase RNF144B; IBR domain-containing protein 2; RING finger protein 144B; p53-inducible RING finger protein

Raised in

Rabbit

Species Reactivity

Human,Mouse

Immunogen

Fusion protein of Human RNF144B

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

Antigen affinity purification

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Form

Liquid

Tested Applications

ELISA,WB,IHC

Recommended Dilution

Application	Recommended Dilution
ELISA	1:2000-1:5000
WB	1:200-1:1000
IHC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates such as LCMT2, thereby promoting their degradation. Induces apoptosis via a p53/TP53-dependent but caspase-independent mechanism. However, its overexpression also produces a decrease of the ubiquitin-dependent stability of BAX, a pro-apoptotic protein, ultimately leading to protection of cell death; But, it is not an anti-apoptotic protein per se.

Gene References into Functions

Low RNF144B expression is associated with gastric cancer. PMID: 27524417
findings substantiate PIR2/Rnf144b as a potentially critical component of epithelial homeostasis, acting downstream of DeltaNp63alpha to regulate cellular levels of p21(WAF1/CIP1) and DeltaNp63alpha PMID: 23128396
PIR2, by being induced by TAp73 and degrading DeltaNp73, differentially regulates TAp73/DeltaNp73 stability. PMID: 20615966
PIR2/RNF144B/IBRDC2 regulates the stability of TAp73 and DNp73. PMID: 20615966
Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator) PMID: 20379614
IBRDC2, an IBR-type RING-finger E3 ubiquitin ligase, regulates the levels of Bax and protects cells from unprompted Bax. activation and cell death. PMID: 20300062
p53RFP, a p53-inducible RING-finger protein, regulates the stability of p21WAF1. PMID: 12853982

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Subcellular Location

Mitochondrion membrane; Single-pass membrane protein. Cytoplasm. Note=Mostly cytosololic, accumulates in submitochondrial domains specifically upon apoptosis induction, in synchrony with BAX activation.

Protein Families

RBR family, RNF144 subfamily

Tissue Specificity

Broadly expressed, with lowest levels in brain and thymus, and highest levels detectable in heart, ovary and testis.

Database Links

HGNC: 21578

KEGG: hsa:255488

STRING: 9606.ENSP00000259939

UniGene: Hs.148741

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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