SLC16A3 Antibody

Datasheet

Code	CSB-PA021410LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: PC-3 whole cell lysate, MCF7 whole cell lysate All lanes:SLC16A3 Antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size:50 kDa Observed band size:50 kDa IHC image of CSB-PA021410LA01HU diluted at 1:300 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of HepG2 cell with CSB-PA021410LA01HU at 1:300, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Product Details
Citations
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) SLC16A3 Polyclonal antibody

Uniprot No.

O15427

Target Names

SLC16A3

Alternative Names

MCT 4 antibody; MCT3 antibody; MCT4 antibody; Monocarboxylate transporter 3 antibody; Monocarboxylate transporter 4 antibody; MOT4_HUMAN antibody; SLC16A3 antibody; Solute carrier family 16 member 3 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Monocarboxylate transporter 4 protein (406-465AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The SLC16A3 Antibody (Product code: CSB-PA021410LA01HU) is Non-conjugated. For SLC16A3 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA021410LB01HU	SLC16A3 Antibody, HRP conjugated	ELISA
FITC	CSB-PA021410LC01HU	SLC16A3 Antibody, FITC conjugated
Biotin	CSB-PA021410LD01HU	SLC16A3 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:20-1:200
IF	1:20-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The SLC16A3 antibody CSB-PA021410LA01HU was produced in the rabbit immunized by using the recombinant human MCT4 protein (406-465aa) as the immunogen. The target protein SLC16A3, also known as MCT4, mainly transports lactate and other monocarboxylates across the plasma membrane of cells, which is crucial for regulating cellular metabolism and maintaining extracellular pH.

This rabbit anti-human SLC16A3 polyclonal antibody was tested in the ELISA and WB applications. The non-conjugated IgG got purified by protein G and reached up to 95% in purity. It reacts with the SLC16A3 proteins from human and rat samples and may be used to detect the endogenous levels of SLC16A3 protein.

Citations

Targeting metabolism by B-raf inhibitors and diclofenac restrains the viability of BRAF-mutated thyroid carcinomas with Hif-1α-mediated glycolytic phenotype M Aprile,British journal of cancer,2023

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Target Background

Function

Proton-linked monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate.

Gene References into Functions

MCT1 may be acting as an uptake transporter and MCT4 as an efflux system across the basolateral membrane for ferulic acid, and that this process is stimulated by butyric acid. PMID: 26854723
Ocular absorption of monocarboxylic acid drugs may be enhanced by MCT transporter SLC16A3, and the absorption route provided by the transporter may be utilized to improve the bioavailability of topically applied ophthalmic drugs. PMID: 20035863
Gamma-hydroxybutyric acid (GHB) is a substrate for both MCT2 and MCT4. PMID: 17502341

Subcellular Location

Cell membrane; Multi-pass membrane protein.

Protein Families

Major facilitator superfamily, Monocarboxylate porter (TC 2.A.1.13) family

Tissue Specificity

Highly expressed in skeletal muscle.

Database Links

HGNC: 10924

OMIM: 603877

KEGG: hsa:9123

STRING: 9606.ENSP00000376150

UniGene: Hs.500761

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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