TRIM13 Antibody

Datasheet

Code	CSB-PA024450ESR2HU
Size	US$166
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Image	Western blot All lanes: TRIM13 antibody at 1.44µg/ml Lane 1: 293T whole cell lysate Lane 2: Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 47, 20, 48 kDa Observed band size: 47 kDa Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA024450ESR2HU at dilution of 1:100 Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA024450ESR2HU at dilution of 1:100
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) TRIM13 Polyclonal antibody

Uniprot No.

O60858

Target Names

TRIM13

Alternative Names

TRIM13; LEU5; RFP2; RNF77; E3 ubiquitin-protein ligase TRIM13; B-cell chronic lymphocytic leukemia tumor suppressor Leu5; Leukemia-associated protein 5; Putative tumor suppressor RFP2; RING finger protein 77; RING-type E3 ubiquitin transferase TRIM13; Ret finger protein 2; Tripartite motif-containing protein 13

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human E3 ubiquitin-protein ligase TRIM13 protein (40-310AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:20-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The immunization of rabbits with the recombinant human TRIM13 protein (40-310AA) and final purification from rabbit antiserum by antigen affinity purification causes the creation of the anti-TRIM13 antibody. This TRIM13 antibody is a polyclonal antibody and exists as an unconjugated IgG. It only shows reactivity with human TRIM13 protein, a RING-type E3 ubiquitin ligase that ubiquitinates L-type channels and induces proteasomal degradation. The TRIM13 protein also enhances apoptosis through protein kinase B, modulates endoplasmic reticulum stress-caused autophagy, affects tumor cell growth, and participates in the regulation of melanoma differentiation-related antigen 5-mediated type I interferon generation. And this anti-TRIM13 antibody has been validated for use in ELISA, WB, and IHC applications.

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Target Background

Function

Endoplasmic reticulum (ER) membrane anchored E3 ligase involved in the retrotranslocation and turnover of membrane and secretory proteins from the ER through a set of processes named ER-associated degradation (ERAD). This process acts on misfolded proteins as well as in the regulated degradation of correctly folded proteins. Enhances ionizing radiation-induced p53/TP53 stability and apoptosis via ubiquitinating MDM2 and AKT1 and decreasing AKT1 kinase activity through MDM2 and AKT1 proteasomal degradation. Regulates ER stress-induced autophagy, and may act as a tumor suppressor. Plays also a role in innate immune response by stimulating NF-kappa-B activity in the TLR2 signaling pathway. Ubiquitinates TRAF6 via the 'Lys-29'-linked polyubiquitination chain resulting in NF-kappa-B activation. Participates as well in T-cell receptor-mediated NF-kappa-B activation. In the presence of TNF, modulates the IKK complex by regulating IKBKG/NEMO ubiquitination leading to the repression of NF-kappa-B.

Gene References into Functions

Results from a study on gene expression variability markers in early-stage human embryos shows that TRIM13 is a putative expression variability marker for the 3-day, 8-cell embryo stage. PMID: 26288249
TRIM13 mediated NF-kappaB repression is essential for negative regulation of clonogenic ability of the cells. PMID: 25152375
Results show that HRD1 and RFP2 contributes are required for the disposal of V247M alpha-sarcoglycan mutant. PMID: 24565866
This study first time demonstrated the role of TRIM13 as novel regulator of caspase-8 activation and cell death during endoplasmic reticulum stress. PMID: 24021263
Shows important roles of TRIM13 in MM tumour survival and proliferation in multiple myeloma. PMID: 23647456
Results describe the role of TRIM13 in induction of autophagy, which may play an important role in regulation of ER stress and tumor suppression. PMID: 22178386
Data suggest that irradiation causes RFP2 overexpression, which enhances ionizing radiation-induced apoptosis by increasing p53 stability and decreasing AKT kinase activity through MDM2 and AKT degradation. PMID: 21333377
Rfp2 contains a C-terminal transmembrane domain indispensable for its localization to the ER PMID: 17314412
TRIM13, also known as RFP2, promoter area contains multiple quadruplex forming GGGGA-repeats that possesses unusually hight activity, anomalously high electrostatic fields induced by sequence-dependent dipoles and very low nucleosome forming potential. PMID: 16499869

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Subcellular Location

Endoplasmic reticulum membrane; Single-pass membrane protein. Note=Concentrates and colocalizes with p62/SQSTM1 and ZFYVE1 at the perinuclear endoplasmic reticulum.

Protein Families

TRIM/RBCC family

Database Links

HGNC: 9976

OMIM: 605661

KEGG: hsa:10206

STRING: 9606.ENSP00000298772

UniGene: Hs.436922

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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