TRIM39 Antibody

Datasheet

Code	CSB-PA881029LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: A549 whole cell lysate, NIH/3T3 whole cell lysate, Mouse skeletal muscle tissue All lanes: TRIM39 antibody at 3μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 60, 57 kDa Observed band size: 45, 50 kDa IHC image of CSB-PA881029LA01HU diluted at 1:600 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescent analysis of Hela cells using CSB-PA881029LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) TRIM39 Polyclonal antibody

Uniprot No.

Q9HCM9

Target Names

TRIM39

Alternative Names

HGNC:10065 antibody; MGC32984 antibody; RING finger protein 23 antibody; RNF23 antibody; Testis abundant finger protein antibody; Testis-abundant finger protein antibody; TFP antibody; TFP RING FINGER PROTEIN 23 antibody; TRI39_HUMAN antibody; Trim39 antibody; TRIM39B antibody; Tripartite motif containing 39 antibody; Tripartite motif-containing protein 39 antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human E3 ubiquitin-protein ligase TRIM39 protein (310-416AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The TRIM39 Antibody (Product code: CSB-PA881029LA01HU) is Non-conjugated. For TRIM39 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA881029LB01HU	TRIM39 Antibody, HRP conjugated	ELISA
FITC	CSB-PA881029LC01HU	TRIM39 Antibody, FITC conjugated
Biotin	CSB-PA881029LD01HU	TRIM39 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:500-1:1000
IF	1:50-1:500

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

E3 ubiquitin-protein ligase. May facilitate apoptosis by inhibiting APC/C-Cdh1-mediated poly-ubiquitination and subsequent proteasome-mediated degradation of the pro-apoptotic protein MOAP1. Regulates the G1/S transition of the cell cycle and DNA damage-induced G2 arrest by stabilizing CDKN1A/p21. Positively regulates CDKN1A/p21 stability by competing with DTL for CDKN1A/p21 binding, therefore disrupting DCX(DTL) E3 ubiquitin ligase complex-mediated CDKN1A/p21 ubiquitination and degradation.; Regulates the G1/S transition of the cell cycle and DNA damage-induced G2 arrest by stabilizing CDKN1A/p21. Positively regulates CDKN1A/p21 stability by competing with DTL for CDKN1A/p21 binding, therefore disrupting DCX(DTL) E3 ubiquitin ligase complex-mediated CDKN1A/p21 ubiquitination and degradation. Negatively regulates the canonical NF-kappa-B signaling pathway via stabilization of CACTIN in an ubiquitination-independent manner.

Gene References into Functions

TRIM39 negatively regulates the NFkappaB signaling pathway possibly via stabilization of cactin. PMID: 26363554
TRIM39R, but not TRIM39B, regulates type I interferon response PMID: 23707810
TRIM39 has a role in regulating cell cycle progression and the balance between cytostasis and apoptosis after DNA damage via stabilizing p21 PMID: 23213251
analysis of ubiquitylation of p53 by the APC/C inhibitor Trim39 PMID: 23213260
these findings suggest that RNF39 and TRIM39 are involved in the etiology of Behcet's disease. PMID: 20875797
Data suggest that TRIM39 can promote apoptosis signalling through stabilization of MOAP-1. PMID: 19100260

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Subcellular Location

[Isoform 1]: Cytoplasm, cytosol. Mitochondrion. Nucleus.; [Isoform 2]: Nucleus.

Protein Families

TRIM/RBCC family

Tissue Specificity

Ubiquitous; highly expressed in brain, heart, kidney, liver, skeletal muscle, spleen and testis.

Database Links

HGNC: 10065

OMIM: 605700

KEGG: hsa:56658

STRING: 9606.ENSP00000365844

UniGene: Hs.413493

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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