URI1 Antibody

Datasheet

Code	CSB-PA529740LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: U87 whole cell lysate All lanes: URI1 antibody at 5.3µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 60, 52, 57, 54 kDa Observed band size: 52 kDa IHC image of CSB-PA529740LA01HU diluted at 1:300 and staining in paraffin-embedded human salivary gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA529740LA01HU diluted at 1:300 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of HepG2 cells with CSB-PA529740LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) URI1 Polyclonal antibody

Uniprot No.

O94763

Target Names

URI1

Alternative Names

C19orf2 antibody; Chromosome 19 open reading frame 2 antibody; NNX3 antibody; NNX3 protein antibody; PPP1R19 antibody; Protein NNX3 antibody; Protein phosphatase 1 regulatory subunit 19 antibody; RMP antibody; RMP_HUMAN antibody; RNA polymerase II subunit 5 mediating protein antibody; RNA polymerase II subunit 5-mediating protein antibody; RPB5 mediating protein antibody; RPB5-mediating protein antibody; Unconventional prefoldin RPB5 interactor 1 antibody; Unconventional prefoldin RPB5 interactor antibody; URI antibody; URI1 antibody; URI1, prefoldin-like chaperone antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Unconventional prefoldin RPB5 interactor 1 protein (180-298AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The URI1 Antibody (Product code: CSB-PA529740LA01HU) is Non-conjugated. For URI1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA529740LB01HU	URI1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA529740LC01HU	URI1 Antibody, FITC conjugated
Biotin	CSB-PA529740LD01HU	URI1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Involved in gene transcription regulation. Acts as a transcriptional repressor in concert with the corepressor UXT to regulate androgen receptor (AR) transcription. May act as a tumor suppressor to repress AR-mediated gene transcription and to inhibit anchorage-independent growth in prostate cancer cells. Required for cell survival in ovarian cancer cells. Together with UXT, associates with chromatin to the NKX3-1 promoter region. Antagonizes transcriptional modulation via hepatitis B virus X protein.; Plays a central role in maintaining S6K1 signaling and BAD phosphorylation under normal growth conditions thereby protecting cells from potential deleterious effects of sustained S6K1 signaling. The URI1-PPP1CC complex acts as a central component of a negative feedback mechanism that counteracts excessive S6K1 survival signaling to BAD in response to growth factors. Mediates inhibition of PPP1CC phosphatase activity in mitochondria. Coordinates the regulation of nutrient-sensitive gene expression availability in a mTOR-dependent manner. Seems to be a scaffolding protein able to assemble a prefoldin-like complex that contains PFDs and proteins with roles in transcription and ubiquitination.

Gene References into Functions

Collectively, these findings indicate that RMP promotes Epithelial-mesenchymal transition and hepatocellular carcinoma metastasis through NF-kappaB/CSN2/Snail pathway. These results suggest that RMP and p65 may serve as potential candidates of the targets in the treatment of metastatic hepatocellular carcinoma. PMID: 28423737
These findings suggest that URI1 has properties of a 'non-oncogene' that supports the oncogenic phenotype of those cancer cells that have evolved a dependency on this molecular chaperone system for survival PMID: 27105489
Amplification of 19q12 CCNE1/URI was found in 10.4% (28/270) and was significantly associated with type II endometrial cancer (EC) high grade, advanced FIGO stage, and aberrant tumor supressor p53 expression. PMID: 27582547
that KAP1 phosphorylation is decreased following recruitment of PP2A by URI. PMID: 27780869
RMP status in tumors may be a useful marker in estimating prognosis in patients with hepatocellular carcinoma and in assisting in the selection of patients who are likely to benefit from adjuvant TACE to prevent relapse. PMID: 25605019
High URI1 expression is associated with epithelial ovarian cancer. PMID: 25527175
Results show that URI expression is essential for hepatocarcinogenesis and associated with poor survival. PMID: 25453901
URI may have an important role in the development of multiple myeloma and chemotherapeutic resistance through activation of the IL-6/STAT3 pathway. PMID: 24625985
Higher level of URI/RMP expression in high-grade endometrioid adenocarcinomas compared to tissues of adjacent endometrium or gland suggests a diagnostic and possibly, a prognostic value of URI/RMP in endometrioid adenocarcinoma. PMID: 24228101
Data show that URI is an "addicting" oncogene selectively required for the survival of ovarian cancer cells with increased URI copy number. PMID: 21397856
a component of a signaling pathway that coordinates nutrient availability with gene expression PMID: 14615539
Data show that URI and PP1gamma are components of an S6K1-regulated mitochondrial pathway dedicated to oppose sustained S6K1 survival signaling and to ensure that the threshold for apoptosis is set based on nutrient and growth factor availability. PMID: 17936702

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Subcellular Location

Nucleus. Cytoplasm. Mitochondrion. Cell projection, dendrite. Note=Colocalizes with PFDN2, PFDN4, PPP1CC, RPS6KB1 and STAP1 at mitochondrion.

Protein Families

RNA polymerase II subunit 5-mediating protein family

Tissue Specificity

Ubiquitous. Expressed in ovarian cancers (at protein level). Expressed strongly in skeletal muscle. Expressed weakly in brain, heart, pancreas and in prostate epithelial cells.

Database Links

HGNC: 13236

OMIM: 603494

KEGG: hsa:8725

STRING: 9606.ENSP00000442436

UniGene: Hs.466391

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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