YKT6 Antibody

Code CSB-PA026265LA01HU
Size US$166
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  • Western blot
    All lanes: YKT6 antibody at 12µg/ml + A549 whole cell lysate
    Secondary
    Goat polyclonal to rabbit IgG at 1/10000 dilution
    Predicted band size: 23, 19 kDa
    Observed band size: 23 kDa

  • Immunohistochemistry of paraffin-embedded human adrenal gland tissue using CSB-PA026265LA01HU at dilution of 1:100

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) YKT6 Polyclonal antibody
Uniprot No.
Target Names
YKT6
Alternative Names
R SNARE antibody; SNARE protein Ykt6 antibody; Synaptobrevin homolog YKT6 antibody; YKT 6 antibody; YKT6 antibody; YKT6 S. cerevisiae homolog of antibody; YKT6 v SNARE homolog antibody; YKT6 v SNARE protein antibody; YKT6_HUMAN antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human Synaptobrevin homolog YKT6 protein (10-195AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The YKT6 Antibody (Product code: CSB-PA026265LA01HU) is Non-conjugated. For YKT6 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA026265LB01HU YKT6 Antibody, HRP conjugated ELISA
FITC CSB-PA026265LC01HU YKT6 Antibody, FITC conjugated
Biotin CSB-PA026265LD01HU YKT6 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
IHC 1:20-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

The production process of the YKT6 polyclonal antibody typically involves injecting the rabbit with the recombinant human synaptobrevin homolog YKT6 protein (10-195aa), collecting its serum, and isolating and purifying the antibody through protein G affinity chromatography.

The YKT6 polyclonal antibody is only reactive with the YKT6 protein from human samples. This antibody contains few impurities, claiming up to 95% purity. It has been tested in ELISA, WB, and IHC. It is commonly used as a tool to detect, quantify, and localize the YKT6 protein in various tissues and cell types.

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Target Background

Function
Vesicular soluble NSF attachment protein receptor (v-SNARE) mediating vesicle docking and fusion to a specific acceptor cellular compartment. Functions in endoplasmic reticulum to Golgi transport; as part of a SNARE complex composed of GOSR1, GOSR2 and STX5. Functions in early/recycling endosome to TGN transport; as part of a SNARE complex composed of BET1L, GOSR1 and STX5. Has a S-palmitoyl transferase activity.
Gene References into Functions
  1. YKT6 is a key molecule in the regulation of exosome release in lung cancer cells and is in turn precisely regulated by miR-134 and miR-135b. Moreover, YKT6 levels impact prognosis of resected Non small cell lung cancer patients. PMID: 27285987
  2. abnormalities in the SNARE complex could represent a molecular substrate for abnormalities of neural connectivity in severe mental disorders PMID: 11884350
  3. Data suggest that GS15 exists in a distinct SNARE complex that contains syntaxin5, GS28, and Ykt6, and may be involved in both ER-to-Golgi and intra-Golgi transport. PMID: 12388752
  4. N-ethylmaleimide-sensitive factor-dependent SNARE-priming complex disassembly is required for efficient exocytosis from mast cells. PMID: 14607937
  5. mRNA expression of CCT5, RGS3, and YKT6 was significantly up-regulated in p53-mutated tumors and associated with a low response rate to docetaxel. PMID: 16821082

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Subcellular Location
Cytoplasm, cytosol. Cytoplasmic vesicle membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus membrane; Lipid-anchor; Cytoplasmic side. Note=Probably cycles through vesicles between Golgi and endosomes.
Protein Families
Synaptobrevin family
Database Links

HGNC: 16959

OMIM: 606209

KEGG: hsa:10652

STRING: 9606.ENSP00000223369

UniGene: Hs.520794

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