fbpA Antibody

Code CSB-PA18279A0Rb
Size US$166
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  • Western Blot
    Positive WB detected in Recombinant Mycobacterium tuberculosis Antigen 85-A protein
    All lanes: Antigen 85-A antibody at 1:1000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 34 kDa
    Observed band size: 34 kDa
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Product Details

Full Product Name
Rabbit anti-Mycobacterium tuberculosis fbpA Polyclonal antibody
Uniprot No.
Target Names
fbpA
Alternative Names
fbpA antibody; mpt44 antibody; Rv3804c antibody; MTV026.09cDiacylglycerol acyltransferase/mycolyltransferase Ag85A antibody; DGAT antibody; EC 2.3.1.122 antibody; EC 2.3.1.20 antibody; Acyl-CoA:diacylglycerol acyltransferase antibody; Antigen 85 complex A antibody; 85A antibody; Ag85A antibody; Fibronectin-binding protein A antibody; Fbps A antibody
Raised in
Rabbit
Species Reactivity
Mycobacterium tuberculosis
Immunogen
Recombinant Mycobacterium tuberculosis Diacylglycerol acyltransferase/mycolyltransferase Ag85A protein (53-331AA)
Immunogen Species
Mycobacterium tuberculosis
Conjugate
Non-conjugated

The fbpA Antibody (Product code: CSB-PA18279A0Rb) is Non-conjugated. For fbpA Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA18279B0Rb fbpA Antibody, HRP conjugated ELISA
FITC CSB-PA18279C0Rb fbpA Antibody, FITC conjugated
Biotin CSB-PA18279D0Rb fbpA Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, WB
Recommended Dilution
Application Recommended Dilution
WB 1:1000-1:5000
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

The polyclonal antibody against fbpA was developed by CUSABIO through immunizing rabbits with a synthetic peptide that corresponds to amino acids 53-331 of the recombinant Ag85A protein, a Diacylglycerol acyltransferase/mycolyltransferase in Mycobacterium tuberculosis. After immunization, the antiserum from the rabbits was subjected to protein G affinity chromatography to purify the anti-fbpA antibody, which exhibits a purity level of 95%. The antibody is present as an unconjugated IgG.

This fbpA polyclonal antibody only reacts with Mycobacterium tuberculosis fbpA, which mediates triacylglycerol (TAG) formation with long-chain acyl-CoA as the acyl donor and 1,2-dipalmitoyl-sn-glycerol (1,2-dipalmitin) as the acyl acceptor and has a preference for C26:0-CoA over C18:1-CoA. This fbpA antibody is suitable for the detection of fbpA protein in ELISA and WB applications.

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Target Background

Function
The antigen 85 proteins (FbpA, FbpB, FbpC) are responsible for the high affinity of mycobacteria for fibronectin, a large adhesive glycoprotein, which facilitates the attachment of M.tuberculosis to murine alveolar macrophages (AMs). They also help to maintain the integrity of the cell wall by catalyzing the transfer of mycolic acids to cell wall arabinogalactan, and through the synthesis of alpha,alpha-trehalose dimycolate (TDM, cord factor). They catalyze the transfer of a mycoloyl residue from one molecule of alpha,alpha-trehalose monomycolate (TMM) to another TMM, leading to the formation of TDM. FbpA mediates triacylglycerol (TAG) formation with long-chain acyl-CoA as the acyl donor and 1,2-dipalmitoyl-sn-glycerol (1,2-dipalmitin) as the acyl acceptor. It has a preference for C26:0-CoA over C18:1-CoA.
Gene References into Functions
  1. Ag85A protein can induce strong specific humoral and Th1 type cellular immune responses. PMID: 29727142
  2. A screen for protein-protein interactions reveals a functional link between the virulence-associated lipid transporter LprG and the mycolyltransferase antigen 85A. PMID: 28276676
  3. Using a prime and pull approach, lentivector vaccines expressing Ag85A induce immunogenicity but fail to induce protection against Mycobacterium bovis bacillus Calmette-Guerin challenge in mice. PMID: 26095282
  4. The authors show that the enzyme possesses acyl-CoA:diacylglycerol acyltransferase (DGAT) activity in addition to its well-known function as mycolyltransferase. PMID: 21819455
  5. findings revealed 6 proteins with high-affinity for the fbpA promoter, and 7 with specificity the fbpC promoter PMID: 19959397
  6. analysis of enzymatic hydrolysis of trehalose dimycolate releases free mycolic acids during mycobacterial growth in biofilms PMID: 20375425
  7. In addition to immune recognition of Ag85A whole protein, peptide-mapping studies identified four immunogenic Ag85A peptides, which induced Th-1 immune responses in individual sarcoidosis subjects, suggesting a role in sarcoidosis immunopathogenesis. PMID: 17357846

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Subcellular Location
Secreted, cell wall. Cytoplasm.
Protein Families
Mycobacterial A85 antigen family
Database Links

KEGG: mtu:Rv3804c

STRING: 83332.Rv3804c

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