GFP-Tag Monoclonal Antibody

Derived from the bioluminescent jellyfish Aequorea victoria, Green Fluorescent Protein (GFP) and its derivatives have become the gold standard for non-invasive, real-time visualization of protein dynamics in living cells. As a ubiquitous genetic tag, the GFP epitope enables researchers to effortlessly track gene expression, protein localization, and cellular processes without the need for external chromophores.

The Mouse Anti-GFP-Tag Monoclonal Antibody (CSB-MA000164, IgG isotype) is expertly produced via robust hybridoma technology, utilizing highly purified recombinant GFP protein as the immunogen. This process ensures exceptional specificity, monospecificity, and minimal batch-to-batch variation, providing researchers with a dependable reagent for routine studies.

Validated for both Enzyme-Linked Immunosorbent Assay (ELISA) and Western Blotting (WB) applications, this antibody exhibits outstanding sensitivity and high binding avidity. As clearly demonstrated in the accompanying Western Blot analysis, the antibody successfully detects 1µg of GFP fusion protein at impressively high primary antibody working dilutions of 1:5,000 and 1:10,000. The presence of sharp, densely staining bands at both concentrations—completely free from background interference or non-specific artifacts—unequivocally confirms the antibody's superior immunoreactivity and exceptional signal-to-noise ratio. Such potent performance at minimal concentrations not only ensures high fidelity in detection but also offers significant cost-efficiency for high-throughput laboratory applications.

This monoclonal antibody provides a definitive method for verifying GFP-tagged protein expression, accurately assessing molecular weight, and monitoring the efficiency of recombinant protein purification. With its proven reliability and versatility, the GFP-Tag Monoclonal Antibody consistently delivers high-quality results, effectively supporting cutting-edge research in live-cell imaging, protein engineering, and subcellular localization studies.