GST-Tag Monoclonal Antibody

Glutathione S-transferase (GST), derived from the parasitic trematode Schistosoma japonicum, is a widely utilized enzyme that serves as an exceptionally effective affinity tag in molecular biology. Unlike smaller peptide tags, the 26 kDa GST tag is renowned for significantly enhancing the solubility and expression yield of otherwise insoluble or poorly expressed recombinant proteins, particularly in prokaryotic systems. Furthermore, its strong, high-affinity binding to glutathione facilitates straightforward one-step affinity purification.

The Mouse Anti-GST-Tag Monoclonal Antibody (CSB-MA000162, IgG isotype) has been meticulously developed to provide researchers with a highly specific tool for detecting this ubiquitous tag. Produced via rigorous hybridoma technology utilizing purified recombinant GST protein as the immunogen, this antibody ensures exceptional monospecificity and reliable lot-to-lot consistency for reproducible experimental outcomes.

Validated for both Enzyme-Linked Immunosorbent Assay (ELISA) and Western Blotting (WB) applications, this reagent exhibits outstanding sensitivity and binding avidity. As vividly demonstrated in the accompanying Western Blot analysis, the antibody successfully detects 0.5 µg of GST fusion protein at impressively high primary antibody working dilutions of 1:5,000 and 1:10,000. The presence of distinct, sharply defined bands at both concentrations—completely devoid of background interference or non-specific artifacts—unequivocally confirms the antibody's high immunoreactivity and excellent signal-to-noise ratio. Such potent performance, even at a 1:10,000 dilution, not only attests to its exceptional quality but also offers significant cost-efficiency for high-throughput laboratory applications.

This monoclonal antibody serves as an indispensable asset for scientists engaged in protein engineering and recombinant DNA technology. It provides a definitive method for verifying GST-tagged protein expression, accurately assessing molecular weight, and meticulously monitoring the efficiency of affinity purification.