HA-Tag Monoclonal Antibody

The HA tag (Haemagglutinin tag) is a widely utilized epitope tag derived from the surface glycoprotein of the human influenza virus. Comprising a short, 9-amino-acid sequence (YPYDVPDYA), the HA tag has become a cornerstone in modern molecular biology and protein engineering. Its primary advantage lies in its remarkably small size (approximately 1 kDa), which ensures minimal interference with the natural folding, stability, and biological function of the target recombinant protein. This makes it an ideal tool for a vast array of applications, including the tracking of protein expression, the isolation of specific proteins via affinity chromatography, and the critical study of protein-protein interactions through techniques such as co-immunoprecipitation (Co-IP).

The Mouse Anti-HA-Tag Monoclonal Antibody (CSB-MA000181, IgG isotype) is meticulously synthesized to provide high-affinity, monospecific recognition of this viral epitope sequence. Produced via robust hybridoma technology using a synthetic peptide as the immunogen, this antibody guarantees exceptional specificity, lot-to-lot consistency, and reliable performance even in complex sample matrices.

Validated for both ELISA and Western Blot (WB) applications, the antibody exhibits outstanding sensitivity and a high binding affinity. As shown in the Western Blot analysis, the antibody successfully detects the HA recombinant protein at a working dilution of 1:2,000. The distinct, crisp band observed on the membrane, with no visible signs of degradation or background interference, underscores the antibody's excellent immunoreactivity and superior signal-to-noise ratio. This high titer and sensitivity make it an exceptionally cost-effective reagent for routine laboratory use.

This antibody is an indispensable tool for molecular biologists. It serves as a critical reagent for verifying successful HA fusion protein expression, accurately assessing protein molecular weight, and providing a reliable handle for downstream biochemical assays and protein purification workflows.