| Code | CSB-MA000272 |
| Size | US$119 |
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| Application | Recommended Dilution |
|---|---|
| WB | 1:500-1:5000 |
The HSV-Tag (Herpes Simplex Virus tag) is a widely utilized epitope tag in molecular biology, derived from a short peptide sequence found in the coat proteins of the Herpes Simplex Virus. Renowned for its compact size, the HSV tag is frequently fused to the N- or C-terminus of target proteins. Its small footprint minimizes the risk of interfering with the folding, stability, or native biological activity of the recombinant protein. Beyond its utility in affinity chromatography, the HSV-tag serves as an exceptionally reliable handle for immunological detection across various experimental platforms.
The Mouse Anti-HSV-Tag Monoclonal Antibody (CSB-MA000272, IgG isotype) is meticulously developed to provide high-affinity, monospecific recognition of this viral epitope sequence. Produced via robust hybridoma technology using a synthetic peptide as the immunogen, this antibody guarantees exceptional specificity, lot-to-lot consistency, and minimal cross-reactivity with endogenous cellular components.
Validated for both ELISA and Western Blot (WB) applications, the antibody exhibits outstanding sensitivity and a high binding affinity. As demonstrated in the Western Blot analysis, the antibody successfully detects the target recombinant protein harboring the Q72 modification at impressively high working dilutions of 1:5,000 and 1:10,000. The distinct, crisp bands observed on the membrane, with no visible signs of degradation or background interference, underscore the antibody's excellent immunoreactivity and superior signal-to-noise ratio. The consistent and clear detection at both concentrations highlights its remarkable titer, making it an exceptionally cost-effective reagent for routine laboratory use.
This antibody is an indispensable tool for molecular biologists and virologists. It serves as a critical reagent for verifying successful HSV fusion protein expression, accurately assessing protein molecular weight, and providing a reliable handle for downstream biochemical assays and protein purification workflows. With its proven performance, the HSV-Tag Monoclonal Antibody ensures dependable, high-quality results to support cutting-edge research in protein engineering, viral vector production, and functional genomics.
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