Flag Tag Monoclonal Antibody

Code CSB-MA000021M0m
Size US$120
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Image
  • Western Blot
    Positive WB detected in: 10ng Flag Tag fusion protein Flag Tag antibody at 1:100000, 1:200000, 1:400000, 1:800000, 1:1600000
    Secondary
    Goat polyclonal to mouse IgG at 1/50000 dilution
    Predicted band size: 35 kDa
    Observed band size: 35 kDa
     

  • Western Blot
    Positive WB detected in: Flag Tag fusion protein at 50ng, 25ng, 12.5ng, 6.25ng, 3.625ng, 1.5625ng, 0.78ng
    All lanes: Flag Tag antibody at 1:1000
    Secondary
    Goat polyclonal to mouse IgG at 1/50000 dilution
    Predicted band size: 35 kDa
    Observed band size: 35 kDa
     

  • Western Blot
    Positive WB detected in: Flag Tag fusion protein 1, 2, 3, 4 at 50ng
    All lanes: Flag Tag antibody at 1:1000
    Secondary
    Goat polyclonal to mouse IgG at 1/50000 dilution
    Predicted band size: 59.6, 36, 35, 42 kDa
    Observed band size: 59.6, 36, 35, 42 kDa
     

  • Western Blot
    Positive WB detected in: Flag Tag fusion protein at 10ng
    All lanes: Company A, Company B, Company C, Company D, CSB-MA000021M0m antibody at 1:50000, 1:100000, 1:200000, 1:400000, 1:800000, 1:1600000, 1:3200000, 1:6400000
    Secondary
    Goat polyclonal to Mouse IgG at 1/50000 dilution
    Predicted band size: 35 kDa
    Observed band size: 35 kDa
     

  • Western Blot
    Positive WB detected in: Flag Tag fusion protein at 50ng, 25ng, 12.5ng, 6.25ng, 3.125ng, 1.5625ng, 0.78ng, 0.39ng
    All lanes: Company A, Company B, Company C, Company D, CSB-MA000021M0m antibody at 1:1000
    Secondary
    Goat polyclonal to Mouse IgG at 1/50000 dilution
    Predicted band size: 35 kDa
    Observed band size: 35 kDa
     

  • Western Blot
    Positive WB detected in: 5 different recombinant proteins with Flag Tag
    All lanes: Company A, Company B, Company C, Company D, CSB-MA000021M0m antibody at 1:5000
    Secondary
    Goat polyclonal to Mouse IgG at 1/50000 dilution
     

  • Immunoprecipitating Flag Tag in Transfected HEK-293 cell whole cell lysate
    Lane 1: Mouse control IgG instead of CSB-MA000021M0m in Transfected HEK-293 cell whole cell lysate
    Lane 2: CSB-MA000021M0m (1µl) + Transfected HEK-293 cell whole cell lysate (500µg)
    Lane 3: Transfected HEK-293 cell whole cell lysate (20µg)
    For western blotting, the blot was detected with CSB-MA000021M0m at 1:5000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000
     

  • Immunoprecipitating Flag Tag in 293F transfected whole cell lysate
    Lane 1: Mouse control IgG instead of CSB-MA000021M0m in Flag Tag in 293F transfected whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
    Lane 2: Company A (1µl), Company B (1µl), Company C (1µl), Company D (1µl), CSB-MA000021M0m (1µl) + Flag Tag in 293F transfected whole cell lysate (500µg)
    Lane 3: Flag Tag in 293F transfected whole cell lysate (20µg)

  • Immunofluorescence staining of 293T cells transfected with Flag tag by CSB-MA000021M0m at 1:300, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-mouse IgG(H+L). The image on the right is the 293T cells transfected without Flag tag.

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Product Details

Alternative Names
DDK tag, DYKDDDDK, Flag, Flag Tag
Raised in
Mouse
Species Reactivity
All
Immunogen
DYKDDDDK(Flag) synthetic peptide conjugate to KLH
Conjugate
Non-conjugated
Clonality
Monoclonal
Isotype
IgG2a
Clone No.
4G5C8
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IF, IP
Recommended Dilution
Application Recommended Dilution
WB 1:1000-1:10000
IF 1:100-1:300
IP 0.5ul-2ul
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description
Flag Tag monoclonal antibody CSB-MA000021M0m was produced in the mouse immunized by using the DYKDDDDK(Flag) synthetic peptide conjugate to KLH as the immunogen. The Flag tag, a popular short peptide tag (DYKDDDDK), can be fused to the C- terminus or N-terminus of the protein. It is used in recombinant DNA technology and can be used for affinity chromatography and can also be used with other affinity tags.
This Flag Tag Monoclonal Antibody was tested in the WB, IF, IP and ELISA applications. The non-conjugated IgG2a got purified by protein G and reached up to 95% in purity. Flag antibodies can be used to detect and express the expression of Flag-tagged fusion proteins, intracellular localization, and to purify, qualitatively or quantitatively detect Flag fusion-expressed proteins. It doesn’t have species restricted.

Customer Reviews and Q&A

 Customer Reviews
Average Rating:
5.0 - 2 reviews

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Applications : MASS SPECTROSCOPY

Sample type: Human HEK-293T cells

Review: Cells were lysed and immunoprecipitated using monoclonal IgG2b anti-Flag antibody.

By Anonymous

Applications : Western Blot (WB)

Review: Cultured cells were washed twice with cold PBS, then lysed using RIPA lysis buffer. In western blotting assay, anti-Flag was applied from CUSABIO.

By Anonymous

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