GST Monoclonal Antibody

Name: GST Monoclonal Antibody
Brand: CUSABIO
SKU: CSB-MA000031M0m
Price: 120 USD
Availability: InStock
Rating: 5.0 (3 reviews)

Datasheet

Citations (5)

Code	CSB-MA000031M0m
Size	US$120
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Image	Western blot All lanes : Recombinant GST Protein at 30ng/ml Line 1: Mouse Anti-GST monoclonal antibody at 1:1000 Line 2: Mouse Anti-GST monoclonal antibody at 1:2000 Line 3: Mouse Anti-GST monoclonal antibody at 1:5000 Line 4: Mouse Anti-GST monoclonal antibody at 1:10000 Line 5: Mouse Anti-GST monoclonal antibody at 1:20000 Line 6: Mouse Anti-GST monoclonal antibody at 1:40000 Line 7: Mouse Anti-GST monoclonal antibody at 1:80000 Predicted band size : 28 kd Observed band size : 28 kd
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Product Details
Citations
Customer Reviews and Q&A

Product Details

Alternative Names

Glutathione S-Transferase, gst, GST tag

Raised in

mouse

Species Reactivity

All

Immunogen

Recombinant GST Protein

Conjugate

Non-conjugated

Clonality

monoclonal

Isotype

IgG2b

Clone No.

2A81D2

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

liquid

Tested Applications

ELISA, WB

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:80000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The glutathione S-transferase (GST) tag is a widely used fusion peptide tag to facilitate recombinant protein purification, expression detection, and quantitative analysis in prokaryotic protein engineering research. CSB-MA000031M0m is a mouse monoclonal IgG2b antibody raised against recombinant GST protein, purified by Protein G affinity chromatography with purity exceeding 95%. Its stable performance has been validated by 5 independent peer-reviewed publications to guarantee consistent batch-to-batch reproducibility and negligible non-specific binding. Functioning as a sensitive tag detection antibody, it specifically recognizes GST-fusion constructs across all species. Western blot on pure recombinant GST protein generates a single sharp 28 kDa band without visible non-specific bands, maintaining clear detectable signals at serial dilutions ranging from 1:1000 up to 1:80000, showing robust linear signal attenuation as dilution rises. Validated for ELISA and Western blot, this antibody provides a flexible working dilution window to support routine recombinant GST protein screening, quantification, and qualitative identification in prokaryotic expression and molecular interaction experiments.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Citations

Rice stripe virus protein NS3 exploits synergistically insect vector importin and ubiquitin systems to promote viral replication L Zhang, Z Qu, Y Tan, Y Li, X Li, Z Huang,Journal of Integrative Agriculture,2025
Polyubiquitylated rice stripe virus NS3 translocates to the nucleus to promote cytosolic virus replication via miRNA-induced fibrillin 2 upregulation L Zhang,PLoS pathogens,2024
Ubiquitin-Conjugating Enzyme E2 E Inhibits the Accumulation of Rice Stripe Virus in Laodelphax striatellus (Fallén) Y Li,Viruses,2020
The α-tubulin of Laodelphax striatellus mediates the passage of rice stripe virus (RSV) and enhances horizontal transmission Y Li,PLoS Pathog,2020
Emerin suppresses Notch signaling by restricting the Notch intracellular domain to the nuclear membrane B Lee.et al,Biochimica et biophysica acta-molecular cell research,2017

Customer Reviews and Q&A

■ Customer Reviews

Average Rating:

5.0 - 3 reviews

Submit a Review here

Applications : WB

Sample dilution: 1: 3000

Review: The in vitro translated emerin deletion mutants were subjected to GST pull-down assays with purified GST and GST-NICD. Bound proteins were analyzed by western blotting with anti-HA antibody. The purified proteins were subjected to western blot analysis with anti-GST antibody.

By Anonymous

Applications : GST pull-down assay

Review: GST pull-down assays. LsTUB was fused to GST and incubated with viruliferous SBPH extracts or GST (control). Blots were probed with anti-NS3 or anti-GST antibodies.

By Anonymous

Applications : GST Pull-Down Assay

Review: Proteins were then separated by SDS–PAGE and detected using anti-GST and anti-LstrE3 antibodies.

By Anonymous

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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