Sumo tag Monoclonal Antibody

Code CSB-MA000132M0m
Size US$120
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  • Western Blot
    Positive WB detected in: 20ng SUMO-tagged fusion protein with SUMO tag antibody at 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000, 1:64000, 1:128000, 1:256000
    Secondary
    Goat polyclonal to Mouse IgG at 1/5000 dilution
    Predicted band size: 55kd
    Observed band size: 55kd
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Product Details

Alternative Names
SUMO tag, SUMO
Raised in
Mouse
Species Reactivity
N/A
Immunogen
Recombinant sumo tag protein
Conjugate
Non-conjugated
Clonality
Monoclonal
Isotype
IgG1
Clone No.
1A52F8
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, WB
Recommended Dilution
Application Recommended Dilution
WB 1:1000-1:256000
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

The small ubiquitin-like modifier (SUMO) tag is a widely utilized fusion tag for investigating protein post-translational modification, protein-protein interactions, and facilitating recombinant protein expression and affinity purification. CSB-MA000132M0m is a mouse monoclonal IgG1 tag antibody raised against recombinant SUMO tag protein, purified via Protein G affinity chromatography with over 95% purity, delivering consistent lot-to-lot performance and exceptional target specificity with minimal non-specific background interference. As a highly reliable tag detection reagent, it has been rigorously validated in Western blot assays, where it detects a distinct 55 kDa target band from 20 ng of SUMO-tagged fusion protein, producing clean, sharp bands free of obvious non-specific staining across a broad working dilution range from 1:1000 up to 1:256000, demonstrating excellent detection sensitivity and wide concentration flexibility for different experimental setups. Validated for ELISA and Western blot applications, this antibody serves as a dependable tool for detection and quantitative analysis of SUMO-fused recombinant proteins, supporting diverse research workflows in protein biochemistry and post-translational modification studies.

Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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