Myc-Tag Monoclonal Antibody

The Myc tag, derived from the c-Myc protein, is a widely adopted 10-amino-acid epitope tag (EQKLISEEDL) in molecular biology. Its compact size ensures minimal interference with the folding and native function of the fused target protein, making it an exceptionally versatile tool for detecting, purifying, and localizing recombinant proteins across diverse expression systems.

The Mouse Anti-Myc-Tag Monoclonal Antibody (CSB-MA000302, IgG isotype) is specifically engineered to provide high-affinity recognition of this unique peptide sequence. Produced via hybridoma technology using a synthetic peptide as the immunogen, this antibody guarantees exceptional specificity, lot-to-lot consistency, and reliable performance in complex sample matrices. Validated for both ELISA and Western Blot (WB) applications, the antibody exhibits outstanding sensitivity. The Western Blot analysis demonstrates its remarkable immunoreactivity, revealing crisp, distinct bands against the His-MBP-C-Myc recombinant protein at two different working dilutions: 1:5,000 and 1:10,000. The clear signal retention even at the higher dilution (1:10,000) underscores the antibody's high titer and excellent signal-to-noise ratio, making it a highly cost-effective reagent for routine laboratory use.

This antibody is an indispensable tool for molecular biologists working with tagged constructs. It enables reliable confirmation of successful protein expression, accurate assessment of protein molecular weight, and serves as a critical reagent for downstream purification and detection workflows.