SRT-Tag Monoclonal Antibody

The SRT-Tag (Short Peptide Tag) is a minimalist epitope composed of just 10 amino acids (TFIGAIATDT), originally derived from Rickettsia typhi. As a highly versatile tool in protein engineering, this small tag is frequently fused to recombinant proteins to facilitate their detection, quantification, and downstream applications such as immunoprecipitation or affinity purification.

The Mouse Anti-SRT-Tag Monoclonal Antibody (CSB-MA000315, IgG isotype) is specifically developed to provide high-affinity recognition of this unique peptide sequence. Produced via hybridoma technology using a recombinant SRT protein as the immunogen, this antibody ensures exceptional specificity and lot-to-lot consistency.

Validated for both ELISA and Western Blot (WB) applications, the antibody exhibits outstanding sensitivity. The accompanying Western Blot analysis demonstrates its robust performance, revealing crisp, distinct bands against the SRT-recombinant protein at two different working dilutions: 1:5,000 and 1:10,000. The clear signal retention even at the higher dilution (1:10,000) underscores the antibody's high titer and excellent signal-to-noise ratio, making it a highly cost-effective reagent for routine laboratory use.

This antibody is an indispensable tool for molecular biologists working with tagged constructs. It enables reliable confirmation of successful protein expression, accurate assessment of protein molecular weight, and serves as a critical positive control in various biochemical assays. With its proven performance, the SRT-Tag Monoclonal Antibody ensures dependable, high-quality results to support your cutting-edge research in protein characterization and functional studies.