T7-Tag Monoclonal Antibody

The T7 tag is a compact, 11-amino acid peptide sequence (MASMTGGQQMG) derived from the N-terminal 11 residues of the major capsid protein (gp10) of the T7 bacteriophage. This epitope serves as a highly versatile tool in molecular biology, widely utilized for recombinant protein expression and detection. Its small size minimizes interference with the target protein's folding and function, while its strong immunogenicity allows for robust immunological handling.

The Mouse Anti-T7-Tag Monoclonal Antibody (CSB-MA000167, IgG isotype) is meticulously developed to provide researchers with monospecific and reliable recognition of this specific epitope. Generated through rigorous hybridoma technology utilizing a precisely synthesized peptide immunogen, this reagent guarantees exceptional specificity and robust lot-to-lot reproducibility.

Validated for both Enzyme-Linked Immunosorbent Assay (ELISA) and Western Blotting (WB) applications, this antibody exhibits outstanding sensitivity and high binding affinity. As vividly demonstrated in the accompanying Western Blot analysis, the antibody successfully detects 0.5 µg of T7 fusion protein at impressively high working dilutions of 1:3,000, 1:5,000, and 1:10,000. The presence of distinct, intensely staining bands at all tested concentrations—conspicuously devoid of background interference or non-specific artifacts—unequivocally confirms the antibody's superior immunoreactivity and excellent signal-to-noise ratio. Notably, the clear banding even at the 1:10,000 dilution highlights its exceptional potency, translating into significant cost savings and making it an extraordinarily economical and powerful tool for high-throughput laboratory applications.

This monoclonal antibody acts as a definitive reagent for verifying T7-tagged protein expression, accurately assessing molecular weight, and monitoring the efficiency of affinity purification protocols. With its proven reliability and versatility, the T7-Tag Monoclonal Antibody consistently delivers high-fidelity results, effectively supporting groundbreaking research in protein engineering, structural genomics, and related disciplines.