Recombinant Arabidopsis thaliana Aquaporin PIP2-1 (PIP2-1)

Code CSB-CF337370DOA
MSDS
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Source in vitro E.coli expression system
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Product Details

Target Names
PIP2-1
Uniprot No.
Alternative Names
PIP2-1; PIP2A; At3g53420; F4P12.120; Aquaporin PIP2-1; Plasma membrane intrinsic protein 2-1; AtPIP2;1; Plasma membrane intrinsic protein 2a; PIP2a
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
2-287
Target Protein Sequence
AKDVEAVPGEGFQTRDYQDPPPAPFIDGAELKKWSFYRAVIAEFVATLLFLYITVLTVIGYKIQSDTDAGGVDCGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLARKVSLPRALLYIIAQCLGAICGVGFVKAFQSSYYTRYGGGANSLADGYSTGTGLAAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYNKSKPWDDHWIFWVGPFIGAAIAAFYHQFVLRASGSKSLGSFRSAANV
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Water channel required to facilitate the transport of water across cell membrane. Probably involved in root water uptake. Its function is impaired by Hg(2+).
Gene References into Functions
  1. Phosphorylation of AtPIP2;1 Ser121 by BAK1 and/or OST1 is triggered in response to flg22 to activate its water and H2O2 transport activities. PMID: 28784763
  2. PIP2;1 activity is regulated by by Ca(2+) and pH. PMID: 27620834
  3. study reveals that C-terminal phosphorylation of a water channel subunit, PIP2;1, is upregulated by an EIN3EIL1-independent ethylene signaling pathway and its water transport activity is enhanced by phosphorylation at the C-terminal S280 and S283 sites PMID: 26476206
  4. This work supports a model whereby abscissic acid-triggered stomatal closure requires an increase in guard cell permeability to water and possibly hydrogen peroxide, through OST1-dependent phosphorylation of PIP2;1 at Ser-121. PMID: 26163575
  5. PIP2;1 molecules are heterogeneously distributed on the plasma membrane and that clathrin and membrane raft pathways cooperate to mediate the subcellular trafficking of PIP2;1. PMID: 22010034
  6. Co-expression of AtPIP2;1-GFP and AtPIP1;4-mCherry constructs suggested that ER-retained AtPIP2;1-GFP may interact with other PIPs to hamper their trafficking to the PM, thereby contributing to inhibition of root cell hydraulic conductivity PMID: 21182578
  7. analysis of the subcellular trafficking of AtPIP2 PMID: 18234664
  8. Structure-function analysis of plant aquaporin AtPIP2. PMID: 18637793

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Subcellular Location
Cell membrane; Multi-pass membrane protein. Note=A fuzzy intracellular localization is induced by salt (NaCl) treatment.
Protein Families
MIP/aquaporin (TC 1.A.8) family, PIP (TC 1.A.8.11) subfamily
Tissue Specificity
Predominantly expressed in roots and green siliques. Also expressed at lower level above ground and in flower buds.
Database Links

KEGG: ath:AT3G53420

STRING: 3702.AT3G53420.1

UniGene: At.47609

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