Recombinant Rat C-C motif chemokine 7 protein (Ccl7) (Active)

CCL7 (MCP-3) is a potent CC chemokine that recruits monocytes, dendritic cells, and lymphocytes through CCR1, CCR2, and CCR3 signaling, making it a critical mediator in inflammatory cell trafficking. This tag-free recombinant rat CCL7 encompasses the full mature sequence (residues 24–97) and demonstrates confirmed biological activity in a human monocyte chemotaxis bioassay at 10–100 ng/mL, providing a reliable basis for Transwell migration assays, CCR2/CCR3 receptor activation studies, and downstream MAPK or NF-κB signaling pathway analysis. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts that can confound monocyte and macrophage functional readouts, which supports use in sensitive cell-based assays and in vivo rat inflammation models. Purity exceeding 95% by SDS-PAGE, combined with this stringent endotoxin control, also makes the protein appropriate for use as a reference standard in cytokine detection assays or as a coating antigen for antibody validation by ELISA.