Product Details

Full Product Name

Rabbit anti-Drosophila melanogaster (Fruit fly) 14-3-3zeta Polyclonal antibody

Uniprot No.

P29310

Target Names

14-3-3zeta

Alternative Names

14-3-3zeta antibody; 14-3-3 antibody; 14-3-3EZ antibody; leo antibody; THAP antibody; CG1787014-3-3 protein zeta antibody; 14-3-3-like protein antibody; Protein Leonardo antibody

Raised in

Rabbit

Species Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

Recombinant Drosophila melanogaster (Fruit fly) 14-3-3zeta protein

Immunogen Species

Drosophila melanogaster (Fruit fly)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Required in Raf-dependent cell proliferation and photoreceptor differentiation during eye development. Acts upstream of Raf and downstream of Ras, and is essential for viability. Acts as a negative regulator of the slo calcium channel via its interaction with slo-binding protein slob. Inhibits yki activity by restricting its nuclear localization. Binds to and promotes the activity of phosphoinositide 3-kinase Pi3K68D which converts phosphatidylinositol to phosphatidylinositol-3-phosphate and promotes maturation of early endosomes.

Gene References into Functions

Study suggests a novel mechanism of Tor regulation mediated by 14-3-3 interaction with Tctp and Rheb. PMID: 27151460
The Khc73 stalk/14-3-3/NudE pathway defines a physical connection that coordinates the activities of multiple motor proteins to precisely position the spindle. PMID: 23987511
Hpo signaling inhibited Yki nuclear localization and activity by phosphorylating Yki and both isoforms of 14-3-3, 14-3-3varepsilon and 14-3-3zeta, regulate Yki activity through modulating its subcellular localization. PMID: 19900439
in vivo 14-3-3zeta monomer properties and functionality PMID: 19920133
monomeric D14-3-3zeta is capable of modulating dSlo channel activity in this regulatory complex. PMID: 12529354
These observations provide the first direct evidence that a 14-3-3 protein functions as a stress-induced molecular chaperone that dissolves and renaturalizes thermal-aggregated proteins. PMID: 16943323
Results report a bona fide third functional isoform encoded by leo divergent from the other two in structurally and functionally significant areas. PMID: 19665025

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Subcellular Location

Cytoplasm. Early endosome.

Protein Families

14-3-3 family

Tissue Specificity

Predominantly expressed in the ventral nerve cord of the embryo, and in the neural tissues of the head. Also found in the region posterior to the morphogenetic furrow of the eye imaginal disk where cells differentiate as photoreceptors.

Database Links

KEGG: dme:Dmel_CG17870

STRING: 7227.FBpp0087500

UniGene: Dm.4705

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14-3-3zeta Antibody

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