Recombinant Drosophila melanogaster 14-3-3 protein zeta (14-3-3zeta)

Code CSB-YP326879DLU
MSDS
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Source Yeast
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Code CSB-EP326879DLU
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Source E.coli
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Code CSB-EP326879DLU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP326879DLU
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Source Baculovirus
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Code CSB-MP326879DLU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
14-3-3zeta
Uniprot No.
Alternative Names
14-3-3zeta; 14-3-3; 14-3-3EZ; leo; THAP; CG1787014-3-3 protein zeta; 14-3-3-like protein; Protein Leonardo
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-248
Target Protein Sequence
MSTVDKEELV QKAKLAEQSE RYDDMAQAMK SVTETGVELS NEERNLLSVA YKNVVGARRS SWRVISSIEQ KTEASARKQQ LAREYRERVE KELREICYEV LGLLDKYLIP KASNPESKVF YLKMKGDYYR YLAEVATGDA RNTVVDDSQT AYQDAFDISK GKMQPTHPIR LGLALNFSVF YYEILNSPDK ACQLAKQAFD DAIAELDTLN EDSYKDSTLI MQLLRDNLTL WTSDTQGDEA EPQEGGDN
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Required in Raf-dependent cell proliferation and photoreceptor differentiation during eye development. Acts upstream of Raf and downstream of Ras, and is essential for viability. Acts as a negative regulator of the slo calcium channel via its interaction with slo-binding protein slob. Inhibits yki activity by restricting its nuclear localization. Binds to and promotes the activity of phosphoinositide 3-kinase Pi3K68D which converts phosphatidylinositol to phosphatidylinositol-3-phosphate and promotes maturation of early endosomes.
Gene References into Functions
  1. Study suggests a novel mechanism of Tor regulation mediated by 14-3-3 interaction with Tctp and Rheb. PMID: 27151460
  2. The Khc73 stalk/14-3-3/NudE pathway defines a physical connection that coordinates the activities of multiple motor proteins to precisely position the spindle. PMID: 23987511
  3. Hpo signaling inhibited Yki nuclear localization and activity by phosphorylating Yki and both isoforms of 14-3-3, 14-3-3varepsilon and 14-3-3zeta, regulate Yki activity through modulating its subcellular localization. PMID: 19900439
  4. in vivo 14-3-3zeta monomer properties and functionality PMID: 19920133
  5. monomeric D14-3-3zeta is capable of modulating dSlo channel activity in this regulatory complex. PMID: 12529354
  6. These observations provide the first direct evidence that a 14-3-3 protein functions as a stress-induced molecular chaperone that dissolves and renaturalizes thermal-aggregated proteins. PMID: 16943323
  7. Results report a bona fide third functional isoform encoded by leo divergent from the other two in structurally and functionally significant areas. PMID: 19665025

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Subcellular Location
Cytoplasm. Early endosome.
Protein Families
14-3-3 family
Tissue Specificity
Predominantly expressed in the ventral nerve cord of the embryo, and in the neural tissues of the head. Also found in the region posterior to the morphogenetic furrow of the eye imaginal disk where cells differentiate as photoreceptors.
Database Links

KEGG: dme:Dmel_CG17870

STRING: 7227.FBpp0087500

UniGene: Dm.4705

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